Commonly, in stimulated emission depletion (STED) fluorescence nanoscopy, light of a wavelength located at the red tail of the emission spectrum of the dye is used to shrink the effective fluorophore excitation volume and ...

We introduce far-field fluorescence nanoscopy with ordinaryfluorophores based on switching the majority of them to ametastable dark state, such as the triplet, and calculating theposition of those left or those that ...

Spatial resolution in far-field fluorescence microscopy is limited by diffraction to about 200 nm. With the aid of photoswitchable fluorophores, the diffraction barrier has been successfully overcome, allowing unprecedented ...

The theoretically unlimited spatial resolution of fluorescence nanoscopy often comes at the expense of time, contrast and increased dose of energy for recording. Here, we developed MoNaLISA, for Molecular Nanoscale Live ...

By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial ...

Figure Persented: Plasmonics and near-field optical nanoscopy both deal with expanding optics into the subwavelength regime. However, these two fields have so far followed parallel paths of development and only recently ...

Fluorescent nanoscopy approaches have been used to characterize the periodic organization of actin,spectrin and associated proteins in neuronal axons and dendrites. This membrane-associated periodicskeleton (MPS) is conserved ...

In recent years, new concepts have emerged for imaging in a far-field fluorescence microscope with resolution under the diffraction limit. All these concepts bear in common the use of molecular states of the probe to switch ...