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Effect of PEG-induced molecular crowding on β-Gal thermal stability
(Sociedad Argentina de Biofísica, 2019)
The yeast β-galactosidase or lactase [EC 3.2.1.23] (β-Gal) is a soluble enzyme capable of catalyzing lactose hydrolysis into its constitutive monosaccharides: glucose and galactose. This enzyme has a commercial application ...
PEG-induced molecular crowding leads to a relaxed conformation, higher thermal stability and lower catalytic efficiency of Escherichia coli β-galactosidase
(Elsevier Science, 2015-12)
Enzymatic activities were historically assayed in dilute solutions where molecular crowding, molecular confinement and their consequences were not taken into account. Here we report how macromolecular crowding tunes catalytic ...
The role of molecular crowding in long-range metalloprotein electron transfer: Dissection into site- and scaffold-specific contributions
(Pergamon-Elsevier Science Ltd, 2019-01)
Here we report the effect of molecular crowding on long-range protein electron transfer (ET) and disentangle the specific responses of the redox site and the protein milieu. To this end, we studied two different one-electron ...
Ultrasensitivity in (SUPRA)molecularly organized and crowded environments
(Academic Press Ltd - Elsevier Science Ltd, 2001-12)
The ultrasensitive response of biological systems is a more sensitive one than that expected from the classical hyperbola of Michaelis-Menten kinetics, and whose physiological relevance depends upon the range of variation ...
Effect of Polyethylene Glycol-Induced Molecular Crowding on the Enzymatic Activity and Thermal Stability of β-Galactosidase from Kluyveromyces lactis
(American Chemical Society, 2020-08)
Here, we report the effect of polyethylene glycol (PEG6000)-induced molecular crowding (MC) on the catalytic activity and thermal stability of Kluyveromyces lactis β-galactosidase (β-Gal). The β-Gal-catalyzed hydrolysis ...
Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast
(Royal Society of Chemistry, 2018-10)
Water is involved in all aspects of biological activity, both as a solvent and as a reactant. It is hypothesized that intracellular water is in a highly structured state due to the high concentrations of macromolecules in ...
Kinetic and structural analysis of the ultrasensitive behaviour of cyanobacterial adp-glucose pyrophosphorylase
(Portland Press, 2000-08)
The kinetic and (supra)molecular properties of the ultrasensitive behaviour of ADP-glucose pyrophosphorylase (AGPase) from Anabaena PCC 7120 (a cyanobacterium) were exhaustively studied. The response of the enzyme toward ...
A Hopping Mechanism for Cargo Transport by Molecular Motors on Crowded Microtubules
(SPRINGER, 2010)
Most models designed to study the bidirectional movement of cargos as they are driven by molecular motors rely on the idea that motors of different polarities can be coordinated by external agents if arranged into a ...
Small Neutral Crowding Solute Effects on Protein Folding Thermodynamic Stability and Kinetics
(2021-10-28)
Molecular crowding is a ubiquitous phenomenon in biological systems, with significant consequences on protein folding and stability. Small compounds, such as the osmolyte trimethylamineN-oxide (TMAO), can also present ...
Far from the madding crowd: The molecular basis for immunological escape of plasmodium falciparum
Like Thomas Hardy’s famous novel Far from the Madding Crowd, Plasmodium falciparum parasites display their most relevant survival structures (proteins) involved in host cell invasion far away from the immune system’s ...