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Two malic enzymes in Pseudomonas aeruginosa
(American Society of Microbiology, 1973)
Cell-free extract supernatant fluids of Pseudomonas aeruginosa were shown to lack malic dehydrogenase but possess a nicotinamide adenine dinucleotide (NAD) - or NAD phosphate (NADP)-dependent enzymatic activity, with ...
Fumarate and cytosolic pH as modulators of the synthesis or consumption of C4 organic acids through NADP-malic enzyme in Arabidopsis thaliana
(Springer, 2013-01)
Arabidopsis thaliana is a plant species that accumulates high levels of organic acids and uses them as carbon, energy and reducing power sources. Among the enzymes that metabolize these compounds, one of the ...
Identification of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis
(Wiley Blackwell Publishing, Inc, 2011-06)
Two paralogous genes, maeE and citM, that encode putative malic enzyme family members were identified in the Enterococcus faecalis genome. MaeE (41 kDa) and CitM (42 kDa) share a high degree of homology between them (47% ...
Biochemical approaches to C4 photosynthesis evolution studies: the case of malic enzymes decarboxylases
(Springer, 2013-07)
C4 photosynthesis enables the capture of atmospheric CO2 and its concentration at the site of RuBisCO, thus counteracting the negative effects of low atmospheric levels of CO2 and high atmospheric levels of O2 (21 %) on ...
Metabolic regulation of phytoplasma malic enzyme and phosphotransacetylase supports the use of malate as an energy source in these plant pathogens
(Society for General Microbiology, 2014-12)
Phytoplasmas ('Candidatus Phytoplasma') are insect-vectored plant pathogens. The genomes of these bacteria are small with limited metabolic capacities making them dependent on their plant and insect hosts for survival. In ...
Allosteric substrate inhibition of Arabidopsis NAD-dependent malic enzyme 1 is released by fumarate
(Elsevier, 2015-02)
Plant mitochondria can use L-malate and fumarate, which accumulate in large levels, as respiratory substrates. In part, this property is due to the presence of NAD-dependent malic enzymes (NAD-ME) with particular biochemical ...
Plastidial NADP-Malic Enzymes from grasses: unravelling the way to the C4 specific isoforms
(Elsevier France-editions Scientifiques Medicales Elsevier, 2013-02)
Malic enzyme is present in many plant cell compartments such as plastids, cytosol and mitochondria. Particularly relevant is the plastidial isoform that participates in the C4 cycle providing CO2 to RuBisCO in C4 species. ...
NAD-malic enzymes of Arabidopsis thaliana display distinct kinetic Q1 mechanisms that support differences in physiological control
(Portland Press, 2010-09)
The Arabidopsis thaliana genome contains two genes encoding NAD-MEs [NAD-dependent malic enzymes; NAD-ME1 (TAIR accession number At4G13560) and NAD-ME2 (TAIR accession number At4G00570)]. The encoded proteins are localized ...
Chimeric structure of plant malic enzyme family: Different evolutionary scenarios for NAD- and NADP-dependent isoforms
(Frontiers Media, 2018-05)
Malic enzyme (ME) comprises a family of proteins with multiple isoforms located in different compartments of eukaryotic cells. In plants, cytosolic and plastidic enzymes share several characteristics such as NADP specificity ...
Determination of malic acid in real samples by using enzyme immobilized reactors and amperometric detection
(Taylor & Francis Inc, 2004-07-01)
The malate dehydrogenase (MDH) and ascorbate oxidase were immobilized independently, onto silanized controlled porous silica and packed in a tygon tube. The reactors were inserted in the flow system, and the malic acid was ...