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Expression and purification of horseradish peroxidase in insect larvae
(Elsevier, 2008-10)
A strategy for obtaining a high-level expression of horseradish peroxidase isozyme C (HRPC) in insect larvae is herein described. The HRPC-6xHis coding sequence was inserted into the AcNPV genome via the pAcGP67HRPC-6xHis ...
Extractive purification of recombinant peroxidase isozyme c from insect larvae in aqueous two-phase systems
(Elsevier Science, 2012-09)
Aqueous two-phase systems (ATPS) have not yet been applied to purify proteins expressed in insect larvae infected by recombinant baculovirus. This work describes the behavior of typical contaminants in the baculovirus-insect ...
An improved method to obtain antigen-excreting Toxocara canis larvae.
(Elsevier Inc, 2014)
Cross-Reactivity Pattern of Asian and American Human Gnathostomiasis in Western Blot Assays Using Crude Antigens Prepared from Gnathostoma spinigerum and Gnathostoma binucleatum Third-Stage Larvae
(American Society of Tropical Medicine and Hygiene, 2016)
Gnathostomiasis is a zoonotic parasitosis endemic in many Asian and some Latin American countries. Most human infections are caused by Gnathostoma spinigerum in Asia and Gnathostoma binucleatum in the Americas, and recently, ...
Horseradish peroxidase production from Spodoptera frugiperda larvae: A simple and inexpensive method
(Elsevier, 2010-06)
Horseradish peroxidase is used in many biotechnological fields including diagnostics, biocatalysts and biosensors. Horseradish peroxidase isozyme C (HRPC) was extracellularly expressed in Spodoptera frugiperda Sf9 cell ...
Integrated process for the purification and immobilization of the envelope protein domain III of dengue virus type 2 expressed in Rachiplusia nu larvae and its potential application in a diagnostic assay
(Academic Press Inc Elsevier Science, 2017-03)
Dengue incidence has grown dramatically in the last years, with about 40% of the world population at risk of infection. Recently, a vaccine developed by Sanofi Pasteur has been registered, but only in a few countries. ...
Purification of Substances from Achyrocline satureioides with Inhibitory Activity Against Paenibacillus larvae, the Causal Agent of American Foulbrood in Honeybees’ Larvae
(Humana Press, 2015-04)
Achyrocline satureioides extracts were tested in vitro against the growth of Paenibacillus larvae. Four different extracts were obtained by liquid-liquid extraction from an aqueous-ethyl alcohol macerate of the aerial parts ...
High-level expression and purification of recombinant wheat germ agglutinin in Rachiplusia nu larvae
(Elsevier, 2015-01)
Wheat germ agglutinin (WGA) is a homodimeric lectin stabilized by non-covalent interactions. Each monomer of 171 residues, which has a complex structure with 16 disulfide bridges, determines two sites for the specific ...
Expression and purification of recombinant feline interferon in the baculovirus-insect larvae system
(Elsevier, 2014-03)
Feline interferons (FeIFNs) are cytokines with antiviral, antitumor and immunomodulatory functions used as therapeutic agents in a variety of veterinary diseases. In this work, FeIFN-α7 and FeIFN-α7xArg containing eight ...