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Interferon-Β lipofection II: Mechanisms involved in cell death and bystander effect induced by cationic lipid-mediated interferon-Β gene transfer to human tumor cells
(Nature Publishing Group, 2012-06)
We evaluated the cytotoxic effects (apoptosis, necrosis and early senescence) of human interferon-B (hIFNB) gene lipofection. The cytotoxicity of hIFNB gene lipofection resulted equivalent to that of the corresponding ...
Interferon-B lipofection I: Increased efficacy of chemotherapeutic drugs on human tumor cells derived monolayers and spheroids
(Nature Publishing Group, 2012-05)
We evaluated the effect of hIFNΒ gene transfer alone or in combination with different antineoplastic drugs commonly used in cancer treatment. Five human tumor-derived cell lines were cultured as monolayers and spheroids. ...
Lipofection of early passages of cell cultures derived from murine adenocarcinomas: In vitro and ex vivo testing of the thymidine kinase/ganciclovir system
(Nature Publishing Group, 2002-12)
Early passages of cultured cells derived from four spontaneous Balb/c murine adenocarcinomas were used to explore the feasibility of a nonviral HSVtk-based suicide gene therapy system. After lipofection with pCMVtk, the ...
Interferon-Β lipofection I: Increased efficacy of chemotherapeutic drugs on human tumor cells derived monolayers and spheroids
(Nature Publishing Group, 2012-06)
We evaluated the effect of hIFNβ gene transfer alone or in combination with different antineoplastic drugs commonly used in cancer treatment. Five human tumor-derived cell lines were cultured as monolayers and spheroids. ...
Cytotoxic effects induced by interferon-ω gene lipofection through ROS generation and mitochondrial membrane potential disruption in feline mammary carcinoma cells
(Academic Press Ltd - Elsevier Science Ltd, 2016-08)
Progress in comparative oncology promises advances in clinical cancer treatments for both companion animals and humans. In this context, feline mammary carcinoma (FMC) cells have been proposed as a suitable model to study ...
Interferon-β gene transfer induces a strong cytotoxic bystander effect on melanoma cells
(Elsevier France-editions Scientifiques Medicales Elsevier, 2015-05)
A local gene therapy scheme for the delivery of type I interferons could be an alternative for the treatment of melanoma. We evaluated the cytotoxic effects of interferon-β (IFNβ) gene lipofection on tumor cell lines derived ...
Lipofection improves gene targeting efficiency in E14 TG2a mouse embryonic stem cells
(Universidad Nacional Autónoma de México, 2016)
Bortezomib enhances the antitumor effects of interferon-β gene transfer on melanoma cells
(Bentham Science Publishers, 2017-05)
We evaluated the cytotoxic effects of the combination of bortezomib (BTZ) and interferon-β (IFNβ) gene lipofection on cultured melanoma cells. Four canine mucosal (Ak, Br, Bk and Ol) and two human dermal (A375 and SB2) ...
Mechanisms enhancing the cytotoxic effects of bleomycin plus suicide or interferon-β gene lipofection in metastatic human melanoma cells
(Bentham Science Publishers, 2018-05)
Background: Three metastatic human melanoma cell lines generated from patient removed lymph nodes and spleen metastasis were established in our laboratory. Objective: To investigate the mechanisms enhancing the cytotoxic ...
Suicide gene therapy on spontaneous canine melanoma: correlations between in vivo tumors and their derived multicell spheroids in vitro
(Nature Publishing Group, 2010-01)
To validate the use of multicellular spheroids to predict the efficacy of herpes simplex thymidine kinase/ganciclovir (HSVtk/GCV) suicide gene therapy in the respective in vivo tumors, we established and characterized 15 ...