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Screening and Selection of Wild Strains for L-arabinose isomerase production
(Brazilian Society of Chemical Engineering, 2013-10)
The majority of L-arabinose isomerases have been isolated by recombinant techniques, but this methodology implies a reduced technological application. For this reason, 29 bacterial strains, some of them previously characterized ...
Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesis
(Molecular Diversity Preservation International, 2017-12)
l-Arabinose isomerase (EC 5.3.1.4) (l-AI) from Enterococcus faecium DBFIQ E36 was overproduced in Escherichia coli by designing a codon-optimized synthetic araA gene. Using this optimized gene, two N- and C-terminal ...
One-Step Immobilization and Stabilization of a Recombinant Enterococcus faecium DBFIQ E36 l-Arabinose Isomerase for d-Tagatose Synthesis
(Humana Press, 2019-04)
A recombinant l-arabinose isomerase from Enterococcus faecium DBFIQ E36 was immobilized onto multifunctional epoxide supports by chemical adsorption and onto a chelate-activated support via polyhistidine-tag, located on ...
Alternative Heterologous Expression of l-Arabinose Isomerase from Enterococcus faecium DBFIQ E36 By Residual Whey Lactose Induction
(Humana Press, 2021-04)
This study reports an alternative strategy for the expression of a recombinant l-AI from Enterococcus faecium DBFIQ E36 by auto-induction using glucose and glycerol as carbon sources and residual whey lactose as inducer ...
Chemical improvement of chitosan-modified beads for the immobilization of Enterococcus faecium DBFIQ E36 l-arabinose isomerase through multipoint covalent attachment approach
(Springer Heidelberg, 2015-10)
d-tagatose is produced from d-galactose by the enzyme l-arabinose isomerase (L-AI) in a commercially viable bioprocess. An active and stable biocatalyst was obtained by modifying chitosan gel structure through reaction ...
Biochemical characterization of heat-tolerant recombinant L-arabinose isomerase from Enterococcus faecium DBFIQ E36 strain with feasible applications in D-tagatose production
(Humana Press, 2019-06)
D-Tagatose is a ketohexose, which presents unique properties as a low-calorie functional sweetener possessing a sweet flavor profile similar to D-sucrose and having no aftertaste. Considered a generally recognized as safe ...
Construction of a surface display system for the expression of L-arabinose isomerase for D-tagatose production
(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2022-06-08)
D-tagatose (D-tag) is a valuable molecule with a high potential for sugar substitution. It is a ketohexose with low caloric value, 90% sweetener than sucrose and acts as a prebiotic. This rare sugar is naturally found in ...
Preparación y caracterización de derivados insolubilizados de la enzima L-arabinosa isomerasa para su empleo en la bioconversión de D-galactosa en D-tagatosa
(2014-06-07)
La D-tagatosa es un azúcar raro, enantiómero de la D-fructosa, que puede obtenerse por isomerización de la D-galactosa. Su gran interés tecnológico reside no sólo en su capacidad endulzante (similar a la sacarosa) y en sus ...