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Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes
(2010)
The intergenic region of spliced-leader (SL-IR) genes from 105 Trypanosoma cruzi I (Tc I) infected biological samples, culture isolates and stocks from 11 endemic countries, from Argentina to the USA were characterised, ...
Geographical clustering of Trypanosoma cruzi I groups from Colombia revealed by low-stringency single specific primer-PCR of the intergenic regions of spliced-leader genes
(Springer, 2009-01)
A low-stringency single-primer polymerase chain reaction (LSSP-PCR) typing procedure targeted to the intergenic regions of spliced-leader genes (SL) was designed to profile Trypanosoma cruzi I stocks from endemic regions ...
Trypanosoma cruzi I haplogroups in american countries based on spliced leader-intergenic spacer polimorphism
(Elsevier, 2010-12)
The intergenic region of spliced-leader (SL-IR) genes from 105 Trypanosoma cruzi I (Tc I) infected biological samples, culture isolates and stocks from 11 endemic countries, from Argentina to the USA were characterised, ...
Characterization of spliced leader genes of Trypanosoma (Megatrypanum) theileri: phylogeographical analysis of Brazilian isolates from cattle supports spatial clustering of genotypes and parity with ribosomal markers
(CAMBRIDGE UNIV PRESS, 2010)
Trypanosoma (Megatrypanum) theileri from cattle and trypanosomes of other artiodactyls form a clade of closely related species in analyses using ribosomal sequences. Analysis of polymorphic sequences of a larger number of ...
Expression of exogenous genes in Trypanosoma cruzi: Improving vectors and electroporation protocols
(Springer, 2004-02)
To improve transfection efficiency in Trypanosoma cruzi, we developed a new electroporation protocol and expression vectors which use luciferase and green and red fluorescent proteins as reporter genes. In transient ...
Padronização da reação de trans-splicing in vitro em tripanosomas utilizando a técnica de RT-PCR
(Universidade Estadual Paulista (UNESP), 2014)
Padronização da reação de trans-splicing in vitro em tripanosomas utilizando a técnica de RT-PCR
(Universidade Estadual Paulista (Unesp), 2014)
Padronização da reação de trans-splicing in vitro em tripanosomas utilizando a técnica de RT-PCR
(Universidade Estadual Paulista (Unesp), 2013-10-16)
The Trypanosomatidae family includes a large number of protozoan parasites, with importants etiological agents of human diseases. Among the causes of diseases stand out Trypanosoma brucei (responsible for sleeping sickness), ...
Preponderant clonal evolution of Trypanosoma cruzi I from Argentinean Chaco revealed by Multilocus Sequence Typing (MLST)
(Elsevier Science, 2014-08-08)
Trypanosoma cruzi has been historically classified as a species with preponderant clonal evolution (PCE). However, with the advent of highly polymorphic markers and studies at geographically reduced scales,the PCE in T. ...
Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients
(Elsevier, 2018)
Background: Chagas disease reactivation is an AIDS defined illness that usually affects Central Nervous System. Gold-Standard diagnosis for T.cruzi reactivation is based on microscopical observation methods.Methods & ...