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Reversible and irreversible fluorescence activity of the Enhanced Green Fluorescent Protein in pH: Insights for the development of pH-biosensors
(2020-12-01)
Enhanced Green Fluorescent Protein (EGFP) is a biomolecule with intense and natural fluorescence, with biological and medical applications. Although widely used as a biomarker in research, its application as a biosensor ...
A Practical Teaching Course in Directed Protein Evolution Using the Green Fluorescent Protein as a Model
(WILEY-BLACKWELL, 2011)
Protein engineering is a powerful tool, which correlates protein structure with specific functions, both in applied biotechnology and in basic research. Here, we present a practical teaching course for engineering the green ...
Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity
(2021-12-01)
Fluorescent Proteins (FP) can be applied as biomarkers and biosensors in the industrial and medical fields, but their large-scale use, especially for new industrial applications, is limited due to their low stability. ...
Citrate and Phosphate Influence on Green Fluorescent Protein Thermal Stability
(JOHN WILEY & SONS LTD, 2011)
Protein structure and function can be regulated by no specific interactions, such as ionic interactions in the presence of salts. Green fluorescent protein (GFP) shows remarkable structural stability and high fluorescence; ...
Effect of Polyethylene Glycol on the Thermal Stability of Green Fluorescent Protein
(JOHN WILEY & SONS LTD, 2010)
Green fluorescent protein (GFP) shows remarkable structural stability and high fluorescence; its stability can be directly related to its fluorescence output, among other characteristics. GFP is stable under increasing ...