Exocytosis of mammalian sperm dense-core secretory granule relies on the same fusion molecules as all other secretory cells; one such molecule is the small GTPase Rab3A. Here, we report an in-depth biochemical characterization ...

The human genome codes for 13 members of a family of filamentforming GTP-binding proteins known as septins. These have been divided into four different subgroups on the basis of sequence similarity. The differences between ...

The role of methylation of elongation factor EF-Tu from Escherichia coli was investigated. The methylated factor was obtained from cells harvested at the late stationary growth phase. Submethylated forms of the factor were ...

When G-protein α subunits binds GTP and Mg2+, they transition from their inactive to their active conformation. This transition is accompanied by completion of the coordination shell of Mg2+ with electrons from six oxygens: ...

Highly purified triads and transverse tubules, as well as a soluble fraction isolated from frog skeletal muscle, hydrolyze exogenous phosphatidylinositol 4,5-bisphosphate forming inositol 1,4,5-trisphosphate with maximal ...

The translation initiation process is an important checkpoint that assures the correct protein production. Within this phase, Initiation Factor IF2 plays an important role along all early and late steps of the process. ...

Bacteria of the genus Prosthecobacter express homologs of eukaryotic alpha- and beta-tubulin, called BtubA and BtubB (BtubA/B), that have been observed to assemble into filaments in the presence of GTP. BtubA/B polymers ...

Arfaptin2 contains a Bin/Amphiphysin/Rvs (BAR) domain and directly interacts with proteins of the Arf/Arl family in their active GTP-bound state. It has been proposed that BAR domains are able to sense membrane ...

The transition of the 30S initiation complex (IC) to the translating 70S ribosome after 50S subunit joining provides an important checkpoint for mRNA selection during translation in bacteria. Here, we study the timing and ...