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Chemical and morphological effects of the contraceptive hormone 17 α-ethynylestradiol on fluid lipid membranes
(2021-08-01)
The lack of studies involving the effects in human health associated with the chronic ingestion of pollutants lead to the path of investigating the action of these compounds in cell membrane models. We demonstrated the ...
Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study
(Elsevier Ireland, 2000-04)
Several methods for the preparation of giant unilamellar vesicles (GUVs) using synthetic phosphatidylcholine phospholipids were evaluated. We compared the physical characteristics - in terms of lamellarity and morphology ...
Lipid domains in giant unilamellar vesicles and their correspondence with equilibrium thermodynamic phases: A quantitative fluorescence microscopy imaging approach
(2009)
We report a novel analytical procedure to measure the surface areas of coexisting lipid domains in giant unilamellar vesicles (GUVs) based on image processing of 3D fluorescence microscopy data. The procedure involves the ...
Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy
(Elsevier Academic Press Inc., 2003-07)
The aim of this chapter is to introduce an experimental approach that allows visualization of temperature-dependent lipid phase equilibria at the level of single vesicles, using two-photon fluorescence microscopy. Various ...