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A genetic and physical study of the interdomain linker of E-Coli AraC protein-a trans-subunit communication pathway
(Wiley, 2016)
Genetic experiments with full length AraC and biophysical experiments with its dimerization domain plus linker suggest that arabinose binding to the dimerization domain changes the properties of the inter-domain linker ...
Rotational diffusion membrane and fluorescence anisotropy.
(Águas de Lindóia, 2014-01-28)
Structural properties of model membranes, such as lipid vesicles, may be investigated through the addition of fluorescent probes. After incorporation, the fluorescent molecules are excited with linearly polarized light and ...
Interaction between 1-pyrenesulfonic acid and albumin: Moving inside the protein
(2019-02-05)
Due to the high sensitivity to alterations in microenvironment polarity of macromolecules, pyrene and its derivatives have long been applied in biosciences. Human serum albumin (HSA), besides its numerous physiological ...
Time-resolved fluorescence and anisotropy-sensitive 1,2-dimyristoyl-sn-glycero-3-(7-aminocoumarin) phosphoetanolamide probe for studying membrane lipid domains
(ELSEVIER SCI LTD, 2023)
A fluorescent probe C-DMPE was synthesised to monitor interfacial membrane properties by conjugating coumarin-343 and 1,2-dimyristoyl-sn-glycero-3-phosphorylethanolamine (DMPE), anchoring the 7-aminocou-marin moiety close ...
Effect of Sonication on the Thermotropic Behavior of DODAB Vesicles Studied by Fluorescence Probe Solubilization
(Springer Heidelberg, 2010-07-01)
The effect of sonication on fluorescence probe solubilization in cationic vesicles of dioctadecyldimethylammonium bromide (DODAB) was investigated by steady-state fluorescence of pyrene (Py), trans-diphenylpolyenes-diphe ...
Effect of Sonication on the Thermotropic Behavior of DODAB Vesicles Studied by Fluorescence Probe Solubilization
(Springer Heidelberg, 2010-07-01)
The effect of sonication on fluorescence probe solubilization in cationic vesicles of dioctadecyldimethylammonium bromide (DODAB) was investigated by steady-state fluorescence of pyrene (Py), trans-diphenylpolyenes-diphe ...
Stereoselective effects of monoterpenes on the microviscosity and curvature of model membranes assessed by fluorescence anisotropy and light scattering analysis.
(Wiley, 2011-11)
Here, we evaluated stereoselectivity in monoterpenes (MTs) ability to disturb membrane dynamics. Correlations between molecular structure and physicochemical properties of pinenes, menthols, and carvones enantiomers were ...
Two-photon anisotropy: Analytical description and molecular modeling for symmetrical and asymmetrical organic dyes
(Elsevier Science BvAmsterdamHolanda, 2006)
Laurdan Spectrum Decomposition as a Tool for the Analysis of Surface Bilayer Structure and Polarity: a Study with DMPG, Peptides and Cholesterol
(SPRINGER/PLENUM PUBLISHERS, 2010)
The highly hydrophobic fluorophore Laurdan (6-dodecanoyl-2-(dimethylaminonaphthalene)) has been widely used as a fluorescent probe to monitor lipid membranes. Actually, it monitors the structure and polarity of the bilayer ...
Laurdan Spectrum Decomposition as a Tool for the Analysis of Surface Bilayer Structure and Polarity: a Study with DMPG, Peptides and Cholesterol
(Springer/plenum Publishers, 2010-03-01)
The highly hydrophobic fluorophore Laurdan (6-dodecanoyl-2-(dimethylaminonaphthalene)) has been widely used as a fluorescent probe to monitor lipid membranes. Actually, it monitors the structure and polarity of the bilayer ...