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Polyelectrolytes–protein complexes: a viable platform in the downstream processes of industrial enzymes at scaling up level
(John Wiley & Sons Ltd, 2016-12)
Complexation between proteins and polyelectrolytes through electrostatic interactions gives either soluble or non-soluble complexes, leading to phase separation. The formation and the stability of these complexes are ...
A new platform for chymotrypsin isolation from fresh bovine pancreas using an environmental friendly polyelectrolyte: Alginate
(Trade Science, 2015-09)
The separation of chymotrypsin from a crude filtrate of fresh bovinepancreas homogenate was carried out using precipitation with acommercially available negatively charged natural weak polyelectrolyte:sodium alginate. The ...
Surfactant presence in a multilayer polyelectrolyte-enzyme system improves its catalytic response
(Elsevier Science Inc, 2009-05)
Self-assembling of poly(allylamine) containing an osmium polypyridil complex (PAOs) alternatively with sodium dodecyl sulfate (SDS) and glucose oxidase (GOx) generates a ternary multilayer system, (PAOs/SDS/PAOs/GOx)n. The ...
Single method of purification for endoglucanase from Aspergillus niger by polyelectrolyte precipitation
(Elsevier, 2016-07)
The precipitation of endoglucanase from Aspergillus niger with synthetic and natural electrically charged polymers -poly vinyl sulfonate (PVS) and chitosan (CHS)- was characterized and applied to a simple method of ...
A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation
(Elsevier Science, 2013-11)
The separation of radish peroxidase from a fresh Raphanus sativus L extract was carried out using precipitation with two commercially available negatively charged synthetic polyelectrolytes: Eudragit® L 100 and Eudragit® ...
Physicochemical study of the formation of complexes between pancreatic proteases and polyanions
(Elsevier Science, 2015-08)
The formation of insoluble complexes between proteins and oppositely charged polyelectrolytes was assessed. Two pancreatic enzymes: trypsin and chymotrypsin, and two anionic synthetic polyelectrolytes: polyacrylate and ...
Multipoint covalent immobilization of lipase on chitosan hybrid hydrogels: influence of the polyelectrolyte complex type and chemical modification on the catalytic properties of the biocatalysts
(Springer Heidelberg, 2011-10)
This work aimed at the production of stabilized derivatives of Thermomyces lanuginosus lipase (TLL) by multipoint covalent immobilization of the enzyme on chitosan-based matrices. The resulting biocatalysts were tested for ...
Chitosan-bovine serum albumin complex formation: A model to design an enzyme isolation method by polyelectrolyte precipitation
(Elsevier Science, 2010-06)
Interactions between a model protein (bovine serum albumin-BSA) and the cationic polyelectrolyte, chitosan (Chi), have been characterized by turbidimetry, circular dichroism and fluorescence spectroscopy. It has been found ...
Multipoint covalent immobilization of lipase on chitosan hybrid hydrogels: influence of the polyelectrolyte complex type and chemical modification on the catalytic properties of the biocatalysts
(SPRINGER HEIDELBERG, 2011)
This work aimed at the production of stabilized derivatives of Thermomyces lanuginosus lipase (TLL) by multipoint covalent immobilization of the enzyme on chitosan-based matrices. The resulting biocatalysts were tested for ...