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Protein-DNA recognition specificity on a model system reveals the complexity and combined role of DNA shape and DNA base readout mechanisms
(2017)
The specific recognition of DNA binding sites by transcription factors (TFs) is key for the
regulation of gene expression. However, most of TFs target short DNA sites and higher
specificity is normally achieved by the ...
Complete genome sequence of bartonella bacilliformis strain KC584 (ATCC 35686)
(American Society for Microbiology, 2020)
Bartonella bacilliformis is the biological agent of Carrion's disease, a vector-borne, life-threatening human bartonellosis restricted to South America. Here, we report the complete genome sequence of B. bacilliformis KC584 ...
Measuring human DNA degradation and gender detection in forensic DNA samples by q-PCR/HRM analysis
(Elsevier Science, 2017-12)
Human DNA quantification, DNA degradation assessment and gender determination are key aspects in most field of human DNA analysis. The assay reported here is a tri-plex Real Time quantitative PCR reaction followed by high ...
DNA staining method based on formazan precipitation induced by blue light exposure
(Journal of Visualized Experiments, 2018)
DNA staining methods are very important for biomedical research. We designed a simple method that allows DNA visualization to the naked eye by the formation of a colored precipitate. It works by soaking the acrylamide or ...
New Perspectives in Mass Disaster Victim Identification Assisted by DNA Typing and Forensic Genomics
(Academic Press Inc Elsevier Science, 2017)
Mass disasters represent a challenging forensic task when victims need to be identified. A wide variety of multiple scenarios force a systematic classification of disasters. The retrieval of human remains, leading to the ...
Structure, function and evolution of the animal mitochondrial replicative DNA helicase
(Taylor & Francis Ltd, 2016-01-02)
The mitochondrial replicative DNA helicase is essential for animal mitochondrial DNA (mtDNA) maintenance. Deleterious mutations in the gene that encodes it cause mitochondrial dysfunction manifested in developmental delays, ...
Adaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNA
(Plant Molecular Biology Reporter, 2018)
Electrochemical nucleic acid biosensors for the detection of interaction between peroxynitrite and DNA
(WILEY-V C H VERLAG GMBH, 2007-07)
We studied the reactivity of peroxynitrite and different nucleic acid molecules using DNA electrochemical biosensors. SIN-1 (3-morpholinosydnonimine) has been used for the simultaneous generation of NO- and superoxide, ...