Buscar
Mostrando ítems 1-10 de 1171
Protein-DNA recognition: towards a new perspective in the definition, analysis and interpretation of protein-DNA binding specificity
(2015)
Protein-DNA binding specificity is currently represented using two-dimensional DNA
sequence logos. These logos contain the frequency of occurrence of the four
nucleotides at each position of the binding site. Based on this ...
Protein-DNA recognition specificity on a model system reveals the complexity and combined role of DNA shape and DNA base readout mechanisms
(2017)
The specific recognition of DNA binding sites by transcription factors (TFs) is key for the
regulation of gene expression. However, most of TFs target short DNA sites and higher
specificity is normally achieved by the ...
A Protein–DNA Binding Mechanism Proceeds Through Multi-state or Two-state Parallel Pathways
(Elsevier, 2003-08)
The DNA-binding mechanism of the dimeric C-terminal domain of the papillomavirus E2 protein with its specific DNA target was investigated and shown to proceed through two parallel pathways. A sequential multi-step reaction ...
Generation of sequence-specific, high affinity anti-DNA antibodies.
(American Society for Biochemistry and Molecular Biology, 2001-12)
By taking advantage of the extreme stability of a protein-DNA complex, we have obtained two highly specific monoclonal antibodies against a predetermined palindromic DNA sequence corresponding to the binding site of the ...
A phage display combined with DNA affinity magnetic system can be applied to a screening of DNA binding proteins, such as transcription factors
(Pontificia Universidad Católica de Valparaíso, 2010)
Interaction of the BELL-like protein ATH1 with DNA: Role of homeodomain residue 54 in specifying the different binding properties of BELL and KNOX proteins
(De Gruyter, 2006-01)
We have studied the interaction of the BELL-like Arabidopsis homeodomain protein ATH1 with DNA. Analysis of oligonucleotides selected by the ATH1 homeodomain from a random mixture suggests that ATH1 preferentially binds ...
A viral DNA-binding domain elicits anti-DNA antibodies of different specificities
(Pergamon-Elsevier Science Ltd, 2005-02)
We have previously demonstrated that immunization of normal mice with a defined protein:DNA complex comprising the C-terminal DNA-binding domain of the human papillomavirus E2 protein and its cognate site 35 oligonucleotide, ...
Arginine methylation and binding of Hrp1p to the efficiency element for mRNA 3'-end formation
(1999-02-01)
Hrp1p is a heterogeneous ribonucleoprotein (hnRNP) from the yeast Saccharomyces cerevisiae that is involved in the cleavage and polyadenylation of the 3'-end of mRNAs and mRNA export. In addition, Hrp1p is one of several ...
Arginine methylation and binding of Hrp1p to the efficiency element for mRNA 3'-end formation
(1999-02-01)
Hrp1p is a heterogeneous ribonucleoprotein (hnRNP) from the yeast Saccharomyces cerevisiae that is involved in the cleavage and polyadenylation of the 3'-end of mRNAs and mRNA export. In addition, Hrp1p is one of several ...
The Influence of Solutes on the Enthalpy/Entropy Change of the Actinomycin D Binding to DNA: Hydration, Energy Compensation and Long-Range Deformation on DNA
(Amer Chemical Soc, 2011-07-21)
The effects of the changes in the temperature and in the water chemical potential on the energetic of the actinomycin D (ACTD) interaction with natural DNA are studied. At reduced water chemical potential, induced by the ...