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Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021
(Elsevier Gmbh, 2014-05)
It has been reported that Ensifer meliloti presents a high proportion of monounsaturated fatty acids and has a putative desaturase gene designated as PhFAD12 (National Centre for Biotechnology Information), encoding a ...
Pseudomonas putida Δ9-fatty acid desaturase: Gene cloning, expression, and function in the cationic surfactants stress
(Wiley VCH Verlag, 2019-05)
Pseudomonas putida counteract the fluidizing effect of cationic surfactants decreasing the content of membrane unsaturated fatty acid (UFA). A Δ9-fatty acid desaturase gene (desA) from P. putida was isolated, cloned, and ...
Improved Characterization of Polyunsaturated Fatty Acids Desaturases and Elongases by Co-Expression in Saccharomyces cerevisiae with a Protozoan Acyl-CoA Synthetase
(Wiley VCH Verlag, 2018-05)
Saccharomyces cerevisiae is a valuable host for the expression and characterization of eukaryotic enzymes involved in polyunsaturated fatty acid (PUFA) biosynthesis, such as elongases and desaturases. The yeast allows a ...
Synergistic effect of inhibitors of fatty acid desaturases on Trypanosoma parasites
(Springer, 2013-06)
The pathway for unsaturated fatty acid biosynthesis is essential in trypanosomatid parasites and has been a key target in our work on the discovery and analysis of several inhibitory compounds. Here, we show the effect of ...
C-5(6) Sterol Desaturase from Tetrahymena thermophila: Gene Identification and Knockout, Sequence Analysis, and Comparison to Other C-5(6) Sterol Desaturases
(American Society for Microbiology, 2009-12)
The gene coding for a C-5(6) sterol desaturase in Tetrahymena thermophila, DES5A, has been identified by the knockout of the TTHERM_01194720 sequence. Macronucleus transformation was achieved by biolistic bombardment and ...
Activity of Delta(9)-desaturase enzyme in mammary gland of lactating buffaloes
(Pagepress Publ, 2007-01-01)
The objective of this research was to measure the activity of e-desaturase enzyme in lactating buffaloes. Data from forty lactating Murrah-crossbred buffaloes were collected on five commercial farms located at Sarapui and ...
Activity of Delta(9)-desaturase enzyme in mammary gland of lactating buffaloes
(Pagepress Publ, 2007-01-01)
The objective of this research was to measure the activity of e-desaturase enzyme in lactating buffaloes. Data from forty lactating Murrah-crossbred buffaloes were collected on five commercial farms located at Sarapui and ...
The sterol-C7 desaturase from the ciliate Tetrahymena thermophila is a Rieske oxygenase which is highly conserved in animals
(Oxford University Press, 2013-04-19)
The ciliate Tetrahymena thermophila incorporates sterols from its environment that desaturates at positions C5(6), C7(8), and C22(23). Phytosterols are additionally modified by removal of the ethyl group at carbon 24 (C24). ...
In Silico Analysis of Fatty Acid Desaturase Genes and Proteins in Grasses
(Humana Press, 2017-07-28)
Fatty acid desaturases (FADs) catalyze the introduction of a double bond into acyl chains. Two FAD groups have been identified in plants: acyl-acyl carrier proteins (ACPs) and acyl-lipid or membrane-bound FAD. The former ...