Human DNA quantification, DNA degradation assessment and gender determination are key aspects in most field of human DNA analysis. The assay reported here is a tri-plex Real Time quantitative PCR reaction followed by high ...

We developed a q-PCR technique that simultaneously evaluates the extent of degradation and determines the gender of a human DNA donor. QYDEG HRM is a triplex real-time PCR whose products are analysed by high-resolution ...

Obtaining informative short tandem repeat (STR) profiles from degraded DNA samples is a challenging task usually undermined by locus or allele dropouts and peak-high imbalances observed in capillary electrophoresis (CE) ...

The use of long ssDNA polynucleotides in PCR of degraded DNA samples can discriminate Short Tandem Repeat (STR) genotypes otherwise missed by current commercial kits.

On degraded DNA samples a multiplex PCR mixture containing polynucleotide primers can provide superior genotyping of Short Tandem Repeats (STRs) to the current commercial kits based on standard oligonucleotides.

Mass disasters represent a challenging forensic task when victims need to be identified. A wide variety of multiple scenarios force a systematic classification of disasters. The retrieval of human remains, leading to the ...

After UV irradiation, DNA polymerases specialized in translesion DNA synthesis (TLS) aid DNA replication. However, it is unclear whether other mechanisms also facilitate the elongation of UV-damaged DNA. We wondered if ...

The present study focused on the genetic analysis of a presumptive Bronze Age family burial, with at least six skeletons (Plana Castell, Barcelona, Spain). In order to investigate the individuals’ biological affiliation, ...