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Aqueous Biphasic Systems Composed of Cholinium Chloride and Polymers as Effective Platforms for the Purification of Recombinant Green Fluorescent Protein
(2018-07-02)
Green fluorescent protein (GFP) has excellent properties as a biosensor and biomarker; however, its widespread use is limited by its purification costs. Alternative low-cost purification techniques can overcome this issue. ...
Effects of cholinium-based ionic liquids on Aspergillus niger lipase: Stabilizers or inhibitors
(2019-01-01)
Lipases are well-known biocatalysts used in several industrial processes/applications. Thus, as with other enzymes, changes in their surrounding environment and/or their thermodynamic parameters can induce structural changes ...
Purification of clavulanic acid produced by Streptomyces clavuligerus via submerged fermentation using polyethylene glycol/cholinium chloride aqueous two-phase systems
(2017-10-25)
Clavulanic acid (CA) is an important pharmaceutical compound produced by batch fermentation of Streptomyces clavuligerus. Since, CA is chemically unstable, its downstream processing should be studied to develop more efficient ...
Understanding the role of the hydrogen bond donor of the deep eutectic solvents in the formation of the aqueous biphasic systems
(2020-01-01)
Deep eutectic solvents (DES) have been proposed as phase-forming compounds of aqueous biphasic systems (ABS). However, due to DES nature and the high water content of the ABS, their nature and behavior remains controversial. ...
Enhancing the Biocatalytic Activity of l -Asparaginase Using Aqueous Solutions of Cholinium-Based Ionic Liquids
(2019-12-16)
l-Asparaginase (ASNase) is a high value enzyme for the pharmaceutical and food industries. Although ionic liquids (ILs) have been recognized as promising solvents or additives for biocatalysis, there are very few studies ...
Economic evaluation of the primary recovery of tetracycline with traditional and novel aqueous two-phase systems
(Elsevier B.V., 2018-09-12)
Antibiotics are a key pharmaceutical to inhibit growth or kill microorganisms. They represent a profitable market and, in particular, tetracycline has been listed as an essential medicine by the WHO. Therefore it is important ...
Desenvolvimento de plataformas sustentáveis para estabilização e purificação da Proteína Verde Fluorescente recombinante produzida por Escherichia coli
(Universidade Estadual Paulista (Unesp), 2020-11-26)
A biotecnologia permitiu o desenvolvimento de novas biomoléculas para aplicações médicas e industriais. No entanto, o uso de muitos produtos biológicos notáveis ainda é limitado em grande escala devido às suas baixas ...
Extração e purificação do biofármaco antileucêmico L-asparaginase (ASNase) utilizando sistemas aquosos bifásicos com polímeros e líquidos iônicos
(Universidade Estadual Paulista (Unesp), 2019-03-22)
A L-asparaginase destaca-se como um importante biofármaco utilizado no tratamento de leucemia, além da sua utilização na indústria alimentícia. Seu alto custo de produção se deve principalmente às etapas de purificação, ...
Sistemas de Duas Fases Aquosas: uma ferramenta biocompatível para extração de lipase de interesse biotecnológico
(Universidade Estadual Paulista (Unesp), 2019-04-24)
As lipases são biocatalisadores que têm como função natural a hidrólise de triglicerídeos na interface lipídeo-água, e apresentam também a capacidade de síntese de ésteres. Quando estas enzimas são produzidas de microrganismos, ...