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The presence of acylated ghrelin during in vitro maturation of bovine oocytes induces cumulus cell DNA damage and apoptosis, and impairs early embryo development
(Cambridge University Press, 2017-09)
The aim of this study was to investigate the effects of acylated ghrelin supplementation during in vitro maturation (IVM) of bovine oocytes. IVM medium was supplemented with 20, 40 or 60 pM acylated ghrelin concentrations. ...
High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells
(Springer Heidelberg, 2017-08-29)
The aim of this study was to investigate the cytotoxic and genotoxic effects of high copper (Cu) concentrations on bovine cumulus cells (CCs) cultured in vitro. We evaluated the effect of 0, 120, 240, and 360 μg/dL Cu added ...
The antioxidant dimethylthiourea improves in vitro fertilization efficiency and decreases cumulus cells apoptosis in pigs
(Csiro Publishing, 2019-09)
Abattoir ovaries, which are the main source of oocytes for reproductive biotechnologies, arrive at the laboratory under ischaemic conditions. Reoxygenation generates reactive oxygen species (ROS) in ischaemic tissues, which ...
Analyses of apoptosis and DNA damage in bovine cumulus cells after in vitro maturation with different copper concentrations: consequences on early embryo development
(Cambridge University Press, 2016-11)
The aim of this study was to investigate the influence of copper (Cu) during in vitro maturation (IVM) on apoptosis and DNA integrity of cumulus cells (CC); and oocyte viability. Also, the role of CC in the transport of ...
Ghrelin antagonist D‐Lys3‐GHRP‐6 counteract ghrelin effects in bovine cumulus‐oocytes complexes matured in vitro
(Wiley Blackwell Publishing, Inc, 2021-09)
Ghrelin is a gut hormone related to energy balance and reproductive functions. The aim of this study was to evaluate the effect of ghrelin antagonist D-Lys3-GHRP-6 (GA) as a potential agent that prevents ghrelin effects ...
Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro
(Academic Press Ltd - Elsevier Science Ltd, 2017-05-15)
The aim of the present study was to investigate the effects of eicosapentaenoic acid (EPA) supplementation during in vitro maturation (IVM) of bovine oocytes. The concentrations tested in all experiments were 1 nM, 1 μM, ...
A co-culture system with cumulus oophurus cells improves the quality of bovine embryos produced in vitroUn sistema de co-cultivo con células del cumulus oophurus mejora la calidad de embriones bovinos producidos in vitro
(Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria, 2020)