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Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
(Wiley, 2002-09)
The effect of solids retention time (SRT) on ammonia and nitrite‐oxidizing bacteria was measured by Nitrosomonas oligotropha‐like ammonia monooxygenase A and Nitrospira 16S rDNA competitive polymerase chain reaction assays ...
Bias in Estimations of DNA Content by Competitive Polymerase Chain Reaction
(Academic Press Inc Elsevier Science, 2000-12)
Competitive PCR is a highly sensitive method for specific DNA quantification. Despite the lack of studies related to the accuracy of the method it has been widely used. Here we present a simulation model for competitive ...
Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics
(Fundação Oswaldo Cruz. Instituto Oswaldo Cruz, 2018)
Identificação de Salmonella spp para controle de qualidade em frigorífico suíno e fábrica de embutidos via PCR
(Universidade Tecnológica Federal do ParanáPonta GrossaBrasilDepartamento Acadêmico de AlimentosTecnologia em AlimentosUTFPR, 2012-10-10)
The consumer requeriment markets for meat produtcs quality and safety is growing. The productive sector is in continious adaptation. Salmnoella sp. Stands out as a major pathogenic bacteria in swine production chain. The ...
Aetiological treatment of congenital Chagas' disease diagnosed and monitored by the polymerase chain reaction
(Oxford University Press, 2003-09)
Objectives: This prospective study focused on the evaluation of anti-parasitic therapy in congenital Chagas' disease, diagnosed and monitored by PCR and conventional diagnosis. Materials and methods: We studied 152 children ...
Otimização de técnica de PCR em tempo real para detecção das regiões pol e env dos subtipos B e F de HIV-1 e triagem de seus recombinantes
(Universidade Federal de São Paulo (UNIFESP), 2009-01-28)
Background: The discovery of 42 HIV-1 circulating recombinant forms (CRF) together with the innumerous unique HIV recombinants forms, makes clear the role of genetic recombination for the epidemic. In Brazil, clades B, F, ...
Diagnóstico fitossanitário por PCR em tempo-real: requisitos básicos para validação de métodosReal-time PCR plant health diagnostics: basic requirements for method validation
(Universidade Federal de ViçosaBRDefesa Sanitária Vegetal; Barreiras não alfandegárias e Comércio InternacionalMestrado Profissional em Defesa Sanitária VegetalUFV, 2015)
Catecholamine effects on human melanoma cells evoked by α1-adrenoceptors
(2004-08-01)
The biological effects of catecholamines in mammalian pigment cells are poorly understood. Our previous results showed the presence of α1-adrenoceptors in SK-Mel 23 human melanoma cells. The aims of this work were to (1) ...