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Expression of glycosylated proteins in bacterial system and purification by affinity chromatography
(2018-01-01)
The bacterial expression of glycoproteins has experienced significant progress in recent years, particularly in regard to the production of conjugate vaccines against pathogens. In this case, a protein carrier conjugated ...
Development of an affinity cryogel for one step purification of lysozyme from chicken egg white
(2017-02-15)
In this study, a supermacroporous polyacrylamide cryogel was produced by cryo-polymerization and activated with Tris(hydroxymethyl)aminomethane (Tris-cryogel) to be applied as an affinity ligand for a one step purification ...
Recovery and purification of aprotinin from industrial insulin-processing effluent by immobilized chymotrypsin and negative IMAC chromatographies
(Elsevier Sci LtdOxfordInglaterra, 2002)
Extension of the selection of protein chromatography and the rate model to affinity chromatographyy
(John Wiley & Sons, 2010-08-03)
The rational selection of optimal protein purification sequences, as well as mathematical models that simulate and
allow optimization of chromatographic protein purification processes have been developed for purification ...
Dyes with High Affinity for Diphtheria Toxoid as Promising Affinity Ligands for its Industrial Purification
(Bentham Science Publishers, 2018-04)
Background: Usually, diphteria toxin concentration and purification are performed after detoxification due to its high danger. In this work, an alternative diphteria toxoid (DTx) concentration and purification method by ...
Effect of IDA and TREN chelating agents and buffer systems on the purification of human IgG with immobilized nickel affinity membranes
(Elsevier Science BvAmsterdamHolanda, 2008)
Cyclodextrin Production by Bacillus lehensis Isolated from Cassava Starch: Characterisation of a Novel Enzyme
(Czech Academy Agricultural Sciences, 2014-01-01)
The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification. Enzyme characteristics ...
Cyclodextrin production by bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme
(2014-03-04)
The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification. Enzyme characteristics ...
Preparation of an affinity cryogel column for lysozyme purification
(2017-08-13)
Affinity cryogels were synthesized using tris(hydroxymethyl)aminomethane (Tris) as ligand for specific interactions with the lysozyme (LYZ). The cryogel was produced by cryo-copolymerization at −12°C. A central composite ...