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Can affinity interactions influence the partitioning of glucose-6-phosphate dehydrogenase in two-phase aqueous micellar systems?
(Sociedade Brasileira de Química, 2008)
In this work, we provide an investigation of the role and strength of affinity interactions on the partitioning of the glucose-6-phosphate dehydrogenase in aqueous two-phase micellar systems. These systems are constituted ...
Integrated extraction and purification of soy isoflavones by using aqueous micellar systems
(Elsevier, 2016-12)
In this work, an integration of solid-liquid and liquid-liquid extractions by using aqueous micellar two-phase systems was evaluated as potential tool to purify soy isoflavones. Additionally, the proposed methodology aimed ...
Extraction of clavulanic acid using aqueous two-phase micellar system
(WILEY-BLACKWELL, 2011)
An investigation of clavulanic acid behavior in an aqueous two-phase micellar system employing the surfactants n-decyltetraethylene oxide (C(10)E(4)) and dodecyldimethylamine oxide (DDAO) was carried out. According to the ...
Application of aqueous two-phase micellar system to improve extraction of adenoviral particles from cell lysate
(Wiley-Blackwell, 2018-05-01)
Viral vectors are important in medical approaches, such as disease prevention and gene therapy, and their production depends on efficient prepurification steps. In the present study, an aqueous two-phase micellar system ...
Design of novel aqueous micellar two-phase systems using ionic liquids as co-surfactants for the selective extraction of (bio)molecules
(Elsevier Science, 2014-10)
Aqueous micellar two-phase systems (AMTPS) using surfactants have been widely applied as an advantageous extraction technology for a wide range of (bio)molecules. When composed by two surfactants (the second one used as ...
Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids
(2020-11-01)
Novel aqueous two-phase micellar systems (ATPMS) composed of Pluronic F68, a triblock amphiphilic copolymer, and cholinium-based ionic liquids (ILs) were formulated and applied for separation/purification of curcumin (CCM). ...
Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 2. Nonionic micellar systems
(2019-02-08)
Lipopolysaccharide endotoxins (LPS) are the most common contaminant pyrogenic compounds found in intracellular recombinant biomolecules purified from Gram-negative bacteria, such as Escherichia coli. Thus, the purification ...
Liquid-liquid extraction of commercial and biosynthesized nisin by aqueous two-phase micellar systems
(ELSEVIER SCIENCE INC, 2008)
Nisin is a natural additive for conservation of food, and can also be used as a therapeutic agent. Nisin inhibits the outgrowth of spores, the growth of a variety of Gram-positive and Grain-negative bacteria. In this paper ...
Influence of salts on the coexistence curve and protein partitioning in nonionic aqueous two-phase micellar systems
(Brazilian Society of Chemical Engineering, 2014-12-01)
Aqueous two-phase micellar systems (ATPMS) can be exploited in separation science for the extraction/purification of desired biomolecules. Prior to phase separation the surfactant solution reaches a cloud point temperature, ...
Dextran sulfate/Triton X two-phase micellar systems as an alternative first purification step for clavulanic acid
(2015)
The development of efficient and cost-effective downstream processes is essential for any pharmaceutical products. Clavulanic acid (CA) is a ß-lactamic drug used combined with antibiotics to inhibit bacterial resistance ...