Buscar
Mostrando ítems 1-10 de 1749
Alkaline phosphatases: In silico study on the catalytic effect of conserved active site residues using human placental alkaline phosphatase (plap) as a model protein
(American Chemical Society, 2020-12)
The metalloenzymes from the alkaline phosphatase (AP) superfamily catalyze the hydrolysis and transphosphorylation of phosphate monoesters. The role of several amino acids highly conserved in the active site of this family ...
Purification and characterization of an iron-activated alkaline phosphatase produced by rhizopus microsporus var. Microsporus under submerged fermentation using rye flour
(2020-07-01)
Current researches have been carried out to find microorganisms that can produce enzymes for different biotechnological purposes. Among the enzymes, the microbial phosphatases, responsible for hydrolyzing phosphoric acid ...
Erythrocyte ghost cell-alkaline phosphatase: construction and characterization of a vesicular system for use in biomineralization studies
(Elsevier B.V., 2002-12-23)
Alkaline phosphatase is required for the mineralization of bone and cartilage. This enzyme is localized in the matrix vesicle, which plays a role key in calcifying cartilage. In this paper we standardize a method to ...
Erythrocyte ghost cell-alkaline phosphatase: construction and characterization of a vesicular system for use in biomineralization studies
(Elsevier B.V., 2002-12-23)
Alkaline phosphatase is required for the mineralization of bone and cartilage. This enzyme is localized in the matrix vesicle, which plays a role key in calcifying cartilage. In this paper we standardize a method to ...
Alkaline phosphatase activity in salivary gland cells of Rhodnius neglectus and R-prolixus (Hemiptera, Triatominae)
(Funpec-editora, 2016-01-01)
Alkaline phosphatase activity was detected in salivary gland cells of the Rhodnius neglectus Lent, 1954, and R. prolixus Stal, 1859, vectors of Trypanosoma cruzi Chagas, 1909 (etiological agent of Chagas disease) and T. ...
Thermostable conidial and mycelial alkaline phosphatases from the thermophilic fungus Scytalidium thermophilum
(Nature America Inc, 2001-10-01)
An extracellular (conidial) and an intracellular (mycelial) alkaline phosphatase from the thermophilic fungus Scytalidium thermophilum were purified by DEAE-cellulose and Concanavalin A-Sepharose chromatography. These ...
Thermostable conidial and mycelial alkaline phosphatases from the thermophilic fungus Scytalidium thermophilum
(Nature America Inc, 2001-10-01)
An extracellular (conidial) and an intracellular (mycelial) alkaline phosphatase from the thermophilic fungus Scytalidium thermophilum were purified by DEAE-cellulose and Concanavalin A-Sepharose chromatography. These ...
Culture of osteogenic cells from human alveolar bone: A useful source of alkaline phosphatase
(Elsevier B.V., 2007-11-01)
The aim of this study was to obtain membrane-bound alkaline phosphatase from osteoblastic-like cells of human alveolar bone. Cells were obtained by enzymatic digestion and maintained in primary culture in osteogenic medium ...