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Produção de β-galactosidase por fungos filamentosos: screening, purificação e caracterização bioquímica
(Universidade Estadual Paulista (Unesp), 2020-02-28)
Conhecida por sua importância na indústria de alimentos e no tratamento de pessoas intolerantes a lactose, a enzima β-galactosidase (EC 3.2.1.23) promove a hidrólise da lactose nos monossacarídeos galactose e glicose. ...
Production of β-galactosidase by Trichoderma sp. through solid-state fermentation targeting the recovery of galactooligosaccharides from whey cheese
(2020-01-01)
Aims: Optimization of β-galactosidase production by Trichoderma sp. under solid-state fermentation using wheat bran as solid substrate through an experimental design and its application targeting the recovery of ...
Superactive β-galactosidase inclusion bodies
(Elsevier Science, 2019-01-01)
Bacterial inclusion bodies (IBs) were historically considered one of the major obstacles in protein production through recombinant DNA techniques and conceived as amorphous deposits formed by passive and rather unspecific ...
Otimização da produção de microcápsulas de alginato de sódio e seu efeito sobre a atividade da β-galactosidase
(Universidade Tecnológica Federal do ParanáLondrinaBrasilTecnologia em AlimentosUTFPR, 2017-06-20)
The inability to hydrolyze lactose contained in the milk, called lactose intolerance, affects approximately 75% of the world's population. This condition is caused by the deficiency or absence of the β-galactosidase enzyme ...
β-Galactosidases from a Sequence-Based Metagenome: Cloning, Expression, Purification and Characterization
(MDPI, 2021)
Stabilization ponds are a common treatment technology for wastewater generated by dairy industries. Large proportions of cheese whey are thrown into these ponds, creating an environmental problem because of the large volume ...
Environmental topology and water availability modulates the catalytic activity of beta-Galactosidase entrapped in a nanosporous silicate matrix
(Nature Publishing Group, 2016-11-04)
In the present work we studied the catalytic activity of E. coli β-Gal confined in a nanoporous silicate matrix (Eβ-Gal) at different times after the beginning of the sol-gel polymerization process. Enzyme kinetic experiments ...
Diseño de matrices de quitosano para la inmovilización de beta-galactosidasas y su aplicación en la síntesis de galacto-oligosacáridos
(2015)
The hipothesis of this investigation was: "The chemical modification of the structure of chitosan by the use of different derivatization methologies will allow the development of immobilized β-galactosidase biocatalysts ...
β-Galactosidase at the membrane–water interface: a case of an active enzyme with non-native conformation
(Elsevier Science, 2013-02)
Previously we demonstrated that Escherichia coli beta-galactosidase (β-Gal) binds to zwitterionic lipid membranes improving its catalytic activity. To understand the activation mechanism from the protein perspective, here ...
Effect of Polyethylene Glycol-Induced Molecular Crowding on the Enzymatic Activity and Thermal Stability of β-Galactosidase from Kluyveromyces lactis
(American Chemical Society, 2020-08)
Here, we report the effect of polyethylene glycol (PEG6000)-induced molecular crowding (MC) on the catalytic activity and thermal stability of Kluyveromyces lactis β-galactosidase (β-Gal). The β-Gal-catalyzed hydrolysis ...
Engineering the Performance of Artificial Inclusion Bodies Built of Catalytic β-Galactosidase
(American Chemical Society Inc, 2021-02-01)
One of the most critical bottlenecks in the application of industrial enzymes is the preservation of protein stability throughout the catalytic reaction, which often requires protein engineering and/or process optimization. ...