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Heterologous expression of the antimyotoxic protein DM64 in Pichia pastoris
(Public Library of Science, 2018)
Recombinant Lactococcus lactis fails to secrete bovine chymosine
(Taylor & Francis, 2014-11)
Bovine chymosin is an important milk-clotting agent used in the manufacturing of cheeses. Currently, the production of recombinant proteins by genetically modified organisms is widespread, leading to greatly reduced costs. ...
PMS1 from Arabidopsis thaliana: optimization of protein overexpression in Escherichia coli
(Springer, 2011-02)
One of the major limitations when attempting to obtain detailed biochemical, biophysical and immunological characterization of plant DNA mismatch repair proteins is their extremely low abundance in vivo under normal growth ...
Evaluation of humoral and cellular immune response after heterologous prime-boost immunization against SARS-CoV-2
(Fiocruz/Instituto de Tecnologia em Imunobiológicos, 2021)
Effect of dilution rate and methanol-glycerol mixed feeding on heterologous Rhizopus oryzae lipase production with Pichia pastoris Mut+ phenotype in continuous culture
(2015-05-01)
The induction using substrate mixtures is an operational strategy for improving the productivity of heterologous protein production with Pichia pastoris. Glycerol as a cosubstrate allows for growth at a higher specific ...
High-level production of MSH2 from Arabidopsis thaliana: a DNA mismatch repair system key subunit
(Humana Press, 2011-02)
Biochemical and immunological information concerning DNA mismatch repair proteins from higher plants is currently limited, probably due to their low abundance in vivo. An initial analysis of AtMSH2 gene expression by ...
Strategies to display heterologous proteins on the cell surface of lactic acid bacteria using as anchor the C-terminal domain of Lactobacillus acidophilus SlpA
(Springer, 2020-10)
The surface-layer (S-layer) protein of Lactobacillus acidophilus is a crystalline array of self-assembling subunits, non-covalently bound to the most outer cell wall envelope, which constitutes up to 20% of the total cell ...