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Keloid explant culture: a model for keloid fibroblasts isolation and cultivation based on the biological differences of its specific regions
(Wiley-Blackwell, 2010-10-01)
In vitro studies with keloid fibroblasts frequently present contradictory results. This may occur because keloids present distinct genotypic and phenotypic characteristics in its different regions, such as the peripheral ...
Human breast tumor slices as an alternative approach to cell lines to individualize research for each patient
(Lippincott Williams & Wilkins, 2014)
Hepatitis B virus: A new platform for in vitro studies of infection, replication cycle and pathogenesis
(2020-01-01)
Hepatitis B virus (HBV) research has been hampered by the lack of suitable and reproducible cell culture systems that reliably mimic the viral life cycle. Several infection stages and metabolic aspects related to HBV cycle ...
Synergistic effect of glucose and prolactin on GLUT2 expression in cultured neonatal rat islets
(1997-03-01)
We studied the synergistic effect of glucose and prolactin (PRL) on insulin secretion and GLUT2 expression in cultured neonatal rat islets. After 7 days in culture, basal insulin secretion (2.8 mM glucose) was similar in ...
Importância do co-cultivo com fibroblastos de camundongo 3T3 para estabelecer cultura de suspensão de células epiteliais do limbo humano
(Conselho Brasileiro de Oftalmologia, 2008-10-01)
PURPOSE: To evaluate the importance of the presence of 3T3 fibroblasts for establishing limbal epithelial cultures from cell suspension obtained from corneo-scleral rims (CSR). METHODS: Corneo-scleral rims from different ...
Volatile profiles of human skin cell cultures in different degrees of senescence
(ELSEVIER SCIENCE BV, 2010)
It is known that skin releases volatile organic Compounds to the environment. and also that its emission pattern changes with aging of the skin. It could be considered, that these compounds are intermediaries in cell ...
Isolation, in vitro study, and stem cell markers for type A spermatogonia in a Characiformes species
(2020-07-01)
The objective of this study was to establish a protocol for the characterization, isolation, and culture of type A spermatogonia using specific molecular markers for these cells in fish. To this end, adult Prochilodus ...