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Optimization of pancreatic trypsin extraction in PEG/citrate aqueous two-phase systems
(Elsevier Inc, 2015-02)
Enzyme extraction using aqueous two-phase systems (ATPS) has been increasingly used as a primary recovery technique which integrates the clarification, concentration and partial purification of important biomolecules from ...
Purification of alpha-toxin from Clostridium perfringens type A in PEG-phosphate aqueous two-phase systems: a factorial study
(JOHN WILEY & SONS LTD, 2008)
BACKGROUND: Purification of a-toxin produced by Clostridium perfringens type A in aqueous two-phase systems (ATPS) was studied with a full two-level factorial design on two factors (concentrations of 8000 g mol(-1) PEG and ...
Amphiphilic copolymer aqueous solutions with cholinium ionic liquids as adjuvants: New insights into determination of binodal curves and phase-separation mechanisms
(2020-11-15)
Aqueous two-phase micellar systems (ATPMS) are more than simple amphiphilic copolymer or surfactant aqueous solutions since due to the formation of biphasic regimes, at certain temperatures, these can be successfully used ...
Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids
(2020-11-01)
Novel aqueous two-phase micellar systems (ATPMS) composed of Pluronic F68, a triblock amphiphilic copolymer, and cholinium-based ionic liquids (ILs) were formulated and applied for separation/purification of curcumin (CCM). ...
Interference of some aqueous two-phase system phase-forming components in protein determination by the Bradford method
(ACADEMIC PRESS INC ELSEVIER SCIENCESAN DIEGO, 2012)
The interference of some specific aqueous two-phase system (ATPS) phase-forming components in bovine serum albumin (BSA) determination by the Bradford method was investigated. For this purpose, calibration curves were ...
Design of novel aqueous micellar two-phase systems using ionic liquids as co-surfactants for the selective extraction of (bio)molecules
(Elsevier Science, 2014-10)
Aqueous micellar two-phase systems (AMTPS) using surfactants have been widely applied as an advantageous extraction technology for a wide range of (bio)molecules. When composed by two surfactants (the second one used as ...