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Mostrando ítems 51-60 de 83
Novos peptídeos sintéticos e estudo da interação com membranas modelo: efeito de modificações no N-terminal na atividade lítica e antimicrobiana
(Universidade Estadual Paulista (Unesp), 2014-09-19)
Antimicrobial peptides (AMPs) are components of the innate immune system, which are present in the majority of the living organisms and whose interest is growing due to their potent antimicrobial activity against a broad ...
Síntese, estrutura e atividade de peptídeos derivados de ParD, o antídoto do módulo toxina-antitoxina ParDE
(Universidade Estadual Paulista (Unesp), 2014-12-15)
The ParE-ParD system represents a toxin-antitoxin module of the broad host range plasmid RK2. ParE (103 amino acids) is the toxin, whereas ParD (83 amino acids) constitutes the antidote able to neutralize ParE by forming ...
Characterization of two cysteine proteinases secreted by Fasciola hepatica and demonstration of their kininogenase activity
(Elsevier B.V., 2001-09-03)
We have isolated and purified two cysteine proteinases of molecular weights 25 and 26 kDa, secreted by Fasciola hepatica adult worm. Their 15 N-terminal residues were found to be identical to those of earlier described ...
NEW SUBSTRATES of PAPAIN, BASED ON the CONSERVED SEQUENCE of NATURAL INHIBITORS of the CYSTATIN FAMILY
(Elsevier B.V., 1994-01-01)
A series of peptide substrates with different fluorogenic leaving groups has been synthesized. the peptide moiety in these substrates mimics a highly conserved sequence (QVVAG) in the natural reversible inhibitors of ...
Substrate specificity characterization of recombinant metallo oligo-peptidases thimet oligopeptidase and neurolysin
(Amer Chemical Soc, 2001-04-10)
We report a systematic and detailed analysis of recombinant neurolysin (EC 3.4.24.16) specificity in parallel with thimet oligopeptidase (TOP, EC 3.4.24.15) using Bk sequence and its C- and N-terminal extensions as in human ...
Characterization of the substrate specificity of the major cysteine protease (cruzipain) from Trypanosoma cruzi using a portion-mixing combinatorial library and fluorogenic peptides
(Portland Press, 1997-04-15)
The substrate specificity of the major cysteinyl proteinase of the parasitic protozoan Trypanosoma cruzi (cruzipain) was investigated, by combinatorial replacement of amino acid residues at positions P-5-P'(5), using a ...
Characterization of thiol-, aspartyl-, and thiol-metallo-peptidase activities in Madin-Darby canine kidney cells
(Wiley-Blackwell, 2000-01-01)
We combined fluorogenic substrates or internally quenched fluorescent peptides with specific inhibitors in the pH profile of proleolytic activity experiments in order to detect proteolytic activities in lysates of MDCK ...
Topography of nicotinic acetylcholine receptor membrane-embedded domains
(American Society for Biochemistry and Molecular Biology, 2000-12)
The topography of nicotinic acetylcholine receptor (AChR) membrane-embedded domains and the relative affinity of lipids for these protein regions were studied using fluorescence methods. Intact Torpedo californica AChR ...
Propriedades hidrolíticas de cisteíno-peptidases recombinantes de Tripanossomatídeos
(Universidade Federal de São Paulo (UNIFESP), 2004)
Trypanosoma cruzi cysteine proteinase cruzipain contains a 130-aminoacid C-terminal extension, in addition to the catalytic domain. Natural cruzipain is a complex of isoforms, because of the simultaneous expression of ...
A continuous fluorescent assay for the determination of plasma and tissue angiotensin I-converting enzyme activity
(Associação Brasileira de Divulgação Científica, 2005-06-01)
A continuous assay using internally quenched fluorescent peptides with the general sequence Abz-peptidyl-(Dnp)P-OH (Abz = ortho-aminobenzoic acid; Dnp = 2,4-dinitrophenyl) was optimized for the measurement of angiotensin ...