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Trypanosoma cruzi: An analysis of the minicircle hypervariable regions diversity and its influence on strain typing
(Elsevier, 2008-11)
The current intraspecific nomenclature in Trypanosoma cruzi describes two major lineages, named T. cruzi I and T. cruzi II, and five sublineages within T. cruzi II, named IIa, IIb, IIc, IId and IIe. The polymorphism of ...
Allelic variant analysis of the DRB3 gene by PCR-RFLP in mediterranean buffaloes breed
(2013-12-01)
The DRB3 gene is a member of the mammalian major histocompatibility complex (MHC) class II cluster. Allelic variants of this gene have been associated with bovine leukemia virus infection and mastitis in cattle. In this ...
Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina
(Embrapa Secretaria de Pesquisa e Desenvolvimento, 2022-06-24)
The objective of this work was to identify, by molecular analysis, the species of the genus Helicoverpa in corn, soybean, and lucerne Bt and non-Bt crops, in central Argentina. A total of 1,343 carterpillars were analyzed ...
PCR Restriction Fragment Length Polymorphism Analyses of V. Parahaemolyticus MAM-7 Virulence Gene in Clinical and Environmental Strains
(iMed publ., 2015)
Virulent and non-virulent Vibrio parahaemolyticus
(V. parahaemolyticus) strains coexist together
in seawater. A PCR–restriction fragment length
polymorphism (PCR-RFLP) technique could
differentiate between clinical ...
Detection of the TLR4 1196C > T Polymorphism by Mismatched-Polymerase Chain Reaction Using Plasmid DNA as Internal Control in Restriction Fragment Length Polymorphism Assays
(MARY ANN LIEBERT INC, 2009)
Background: Restriction fragment length polymorphism (RFLP) is a common molecular assay used for genotyping, and it requires validated quality control procedures to prevent mistyping caused by impaired endonuclease activity. ...
Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene
(Instituto Oswaldo Cruz, Ministério da Saúde, 2014)
Multiplex PCR followed by restriction length polymorphism for the subtyping of bovine herpesvirus 5 isolates
(Biomed Central, 2013-06)
Abstract Background: Several types and subtypes of bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) have been associated to different clinical conditions of cattle, making type/subtype differentiation essential to ...
MODY 2: Mutation identification and molecular ancestry in a Brazilian family
(2013-01-10)
Maturity Onset Diabetes of the Young (MODY) is a heterogeneous group of genetic diseases characterized by a primary defect in insulin secretion and hyperglycemia, non-ketotic disease, monogenic autosomal dominant mode of ...