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Effect of follicle size on mRNA expression in cumulus cells and oocytes of Bos indicus: an approach to identify marker genes for developmental competence
(CSIRO Publishing, 2009-01-01)
To identify the genes related to oocyte competence, we quantified transcripts for candidate genes in oocytes (H1Foo, H2A, H3A, GHR, GDF9, BMP15, OOSP1) and cumulus cells (FSHR, EGFR, GHR, PTX3, IGFII) using the follicle ...
Regulação da expressão de fatores secretados pelo oócito (FSOs) e seus receptores durante a maturação in vitro (MIV) bovina e ações no controle da expressão de cumulus
(Universidade Estadual Paulista (Unesp), 2011-02-06)
O oócito participa ativamente dos mecanismos reguladores da maturação do complexo cumulus-oócito (COC) via secreção de fatores parácrinos. A proteína morfogênica óssea 15 (BMP15) e o fator de crescimento e diferenciação 9 ...
Influence of manganese on apoptosis and glutathione content of cumulus cells during in vitro maturation in bovine oocytes
(Academic Press Ltd-elsevier Science Ltd, 2014-02-14)
We have investigated the effect of different Mn concentrations on (1) DNA integrity of cumulus cells by olive tail moment (OTM); (2) cumulus cells apoptosis by Annexin V staining assay; (3) intracellular total glutathione ...
High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells
(Springer Heidelberg, 2017-08-29)
The aim of this study was to investigate the cytotoxic and genotoxic effects of high copper (Cu) concentrations on bovine cumulus cells (CCs) cultured in vitro. We evaluated the effect of 0, 120, 240, and 360 μg/dL Cu added ...
Effect of Different Manganese Concentrations during in vitro Maturation of Bovine Oocytes on DNA Integrity of Cumulus Cells and Subsequent Embryo Development
(Wiley-blackwell Publishing, Inc, 2013-05)
Manganese (Mn) is a trace element present in forages and cereals, and its concentration depends on soil status. Manganese deficiency in cattle, goats and ewes not only impairs oestrous cycle but reduces calf birth weight. ...
Influência da temperatura de fecundação In vitro e das células do Cumulus oophorus sobre a taxa de polispermia e desenvolvimento embrionário
(Goiânia, 2010)
O objetivo deste trabalho foi avaliar o efeito da temperatura (37 ou 38,5°C) de fecundação in vitro (FIV) e da retirada das células do cumulus oophorus após a FIV ou após doze horas de cultivo embrionário (CIV), nos índices ...
Doramectin induced cytotoxic and genotoxic effects on bovine peripheral lymphocytes and cumulus cells in vitro
(Taylor & Francis, 2019-02)
The effect of doramectin (DOR) was tested on two experimental somatic bovine cells in vitro: peripheral lymphocytes (PL) and cumulus cells (CC). The cytotoxicity and genotoxicity of DOR were assessed using 3-(4,5-dimethy ...