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S3 to S3 ' subsite specificity of recombinant human cathepsin K and development of selective internally quenched fluorescent substrates
(Portland Press, 2003-08-01)
We have systematically examined the S3 to S3' subsite substrate specificity requirements of cathepsin K using internally quenched fluorescent peptides derived from the lead sequence Abz-KLRFSKQ-EDDnp [where Abz is ...
Conformational Properties of Angiotensin II and Its Active and Inactive TOAC-Labeled Analogs in the Presence of Micelles. Electron Paramagnetic Resonance, Fluorescence, and Circular Dichroism Studies
(JOHN WILEY & SONS INC, 2009)
The interaction between angiotensin II (AII, DRVYIHPF) and its analogs carrying 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (TOAC) and detergents-negatively charged sodium dodecyl sulfate (SDS) and ...
Interaction of gramicidin with DPPC/DODAB bilayer fragments
(ELSEVIER SCIENCE BVAMSTERDAM, 2012)
The interaction between the antimicrobial peptide gramicidin (Gr) and dipalmitoylphosphatidylcholine (DPPC)/dioctadecyldimethylammonium bromide (DODAB) 1:1 large unilamellar vesicles (LVs) or bilayer fragments (BFs) was ...
Short peptide constructs mimic agonist sites of AT(1)R and BK receptors
(Springer, 2013-03-01)
Extracellular peptide ligand binding sites, which bind the N-termini of angiotensin II (AngII) and bradykinin (BK) peptides, are located on the N-terminal and extracellular loop 3 regions of the AT(1)R and BKRB1 or BKRB2 ...
Analysis of peptidase activities of a cathepsin B-like (TcoCBc1) from Trypanosoma congolense
(Elsevier B.V., 2014-07-01)
The substrate specificity of TcoCBc1 was evaluated using two internally quenched fluorescent peptide libraries with randomized sequences designed to detect carboxydipeptidase (Abz-GXXZXK(Dnp)-OH) and endopeptidase ...
The Mycobacterium leprae hsp65 displays proteolytic activity. Mutagenesis studies indicate that the M. leprae hsp65 Proteolytic activity is catalytically related to the HslVU protease?
(2002-06-11)
The present study reports, for the first time, that the recombinant hsp65 from Mycobacterium leprae (chaperonin 2) displays a proteolytic activity toward oligopeptides. The M. leprae hsp65 proteolytic activity revealed a ...
Permeability changes induced by polylysines in rat spermatids
(2002)
High molecular weight (HMW, >15 kDa) but not low molecular weight (LMW, <15 kDa) polylysines (PLs) bound and induced permeability changes in rat spermatid plasma membranes, estimated by Mn2+ quenching of intracellular ...
Generation and implementation of reporter BHK-21 cells for live imaging of flavivirus infection
(2021-03-05)
The genus Flavivirus within the family Flaviviridae includes many viral species of medical
importance, such as yellow fever virus (YFV), Zika virus (ZIKV), and dengue virus (DENV), among
others. Presently, the identification ...
Chromogenic and fluorogenic glycosylated and acetylglycosylated peptides as substrates for serine, thiol and aspartyl proteases
(Munksgaard Int Publ Ltd, 1999-02-01)
We synthesized short chromogenic peptidyl-Arg-p-nitroanilides containing either (Gal beta)Ser or (Glc alpha,beta)Tyr at P-2 or P-3 sites as well as O-acetylated sugar moieties and studied their hydrolysis by bovine trypsin, ...
Expression and substrate specificity of a recombinant cysteine proteinase B of Leishmania braziliensis
(Elsevier B.V., 2008-10-01)
The cysteine proteinase B of Leishmania parasites is an important virulence factor. in this study we have expressed, isolated and characterized for the first time a recombinant CPB from Leishmania braziliensis, the causative ...