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A continuous fluorescence resonance energy transfer angiotensin I-converting enzyme assay
(Nature Publishing Group, 2006-01-01)
Angiotensin I-converting enzyme (ACE) is involved in various physiological and physiopathological conditions; therefore, the measurement of its catalytic activity may provide essential clinical information. This protocol ...
Fluorescent patterning of paper through laser engraving
(Royal Society of Chemistry, 2020-09)
While thermal treatment of paper can lead to the formation of aromatic structures via hydrothermal treatment (low temperature) or pyrolysis (high temperature), neither of these approaches allow patterning the substrates. ...
Positional-scanning combinatorial libraries of fluorescence resonance energy transfer peptides for defining substrate specificity of the angiotensin I-converting enzyme and development of selective C-domain substrates
(Amer Chemical Soc, 2004-12-21)
Positional-scanning combinatorial libraries of fluorescence resonance energy transfer peptides were used for the analyses of the S-3 to S-1' subsites of the somatic angiotensin I-converting enzyme (ACE). Substrate specificity ...
Measuring elastase, proteinase 3 and cathepsin G activities at the surface of human neutrophils with fluorescence resonance energy transfer substrates
(Nature Publishing Group, 2008-01-01)
The neutrophil serine proteases (NSPs) elastase, proteinase 3 and cathepsin G are multifunctional proteases involved in pathogen destruction and the modulation of inflammatory processes. A fraction of secreted NSPs remains ...
Differences in nucleotide-binding site of isoapyrases deduced from tryptophan fluorescence
(PERGAMON-ELSEVIER SCIENCE LTD, 2003-05)
Comparative studies of intrinsic and extrinsic fluorescence of apyrases purified from two potato tuber varieties (Pimpernel and Desiree) were performed to determine differences in the microenvironment of the nucleotide ...
Plasmon-enhanced fluorescence spectroscopy
(Royal Society Chemistry, 2017)
Fluorescence spectroscopy with strong emitters is a remarkable tool with ultra-high sensitivity for detection and imaging down to the single-molecule level. Plasmon-enhanced fluorescence (PEF) not only offers enhanced ...
Analysis of peptidase activities of a cathepsin B-like (TcoCBc1) from Trypanosoma congolense
(Elsevier B.V., 2014-07-01)
The substrate specificity of TcoCBc1 was evaluated using two internally quenched fluorescent peptide libraries with randomized sequences designed to detect carboxydipeptidase (Abz-GXXZXK(Dnp)-OH) and endopeptidase ...
The use of Fluorescence Resonance Energy Transfer (FRET) peptidesfor measurement of clinically important proteolytic enzymes
(Academia Brasileira de Ciências, 2009-09-01)
Proteolytic enzymes have a fundamental role in many biological processes and are associated with multiple pathological conditions. Therefore, targeting these enzymes may be important for a better understanding of their ...