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Levels of expression and immunogenicity of attenuated Salmonella enterica serovar typhimurium strains expressing Escherichia coli mutant heat-labile enterotoxin
(Amer Soc MicrobiologyWashingtonEUA, 1998)
Selection and validation of reference genes by RT-qPCR under photoperiodic induction of flowering in sugarcane (Saccharum spp.)
(2021-12-01)
Although reference genes have previously been used in the expression analysis of genes involved in sugarcane flowering they had not been experimentally validated for stability and consistency of expression between different ...
Genomic integration as a way to stable high producers CHO cells
(Fiocruz/Instituto de Tecnologia em Imunobiológicos, 2019)
Collision and Stable Regions around Bodies with Simple Geometric Shape
(Hindawi Publishing Corporation, 2009-01-01)
We show the expressions of the gravitational potential of homogeneous bodies with well-defined simple geometric shapes to study the phase space of trajectories around these bodies. The potentials of the rectangular and ...
Collision and Stable Regions around Bodies with Simple Geometric Shape
(Hindawi Publishing Corporation, 2009-01-01)
We show the expressions of the gravitational potential of homogeneous bodies with well-defined simple geometric shapes to study the phase space of trajectories around these bodies. The potentials of the rectangular and ...
Selection and validation of reference genes for functional studies in the Calliphoridae family
(Milwaukee, 2014)
The genera Cochliomyia and Chrysomya contain both obligate and saprophagous flies, which allows the comparison of different feeding habits between closely related species. Among the different strategies for comparing these ...
Murine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryo
(Wiley-Blackwell, 2008-11-01)
We describe two replication incompetent retroviral vectors that co-express green fluorescent protein (GFP) and beta-galactosidase. These vectors incorporate either the avian reticuloendotheliosis (spleen necrosis virus; ...
Cloning and expression of transgenes using linear vectors in Trypanosoma cruzi
(Elsevier, 2014-04)
The identification of new targets for vaccine and drug development for the treatment of Chagas’ disease is dependent on deepening our understanding of the parasite genome. Vectors for genetic manipulation in Trypanosoma ...
Trans-complementation of polyhedrin by a stably transformed Sf9 insect cell line allows occ- baculovirus occlusion and larval per os infectivity
(Elsevier Science, 2010-01)
Oral infection of insect larvae with baculovirus is an advantageous methodology for producing high levels of recombinant proteins and for achieving plague control. However, many recombinant baculoviruses express a foreign ...
Statistical differences resulting from selection of stable reference genes after hypoxia and hypothermia in the neonatal rat brain
(Public Library of Science, 2020-05)
Real-time reverse transcription PCR (qPCR) normalized to an internal reference gene (RG), is a frequently used method for quantifying gene expression changes in neuroscience. Although RG expression is assumed to be constant ...