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Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos
(2019-03-15)
Purpose: Oocyte maturation is a complex process involving nuclear and cytoplasmic modulations, during which oocytes acquire their ability to become fertilized and support embryonic development. The oocyte is apparently ...
Linoleic acid stimulates neutral lipid accumulation in lipid droplets of maturing bovine oocytes
(Elsevier, 2013-03)
Linoleic acid (LA) is a polyunsaturated fatty acid present in high concentrations in bovine follicular fluid; when added to maturation culture media, it affects oocyte competence (depending on the type and concentration ...
Reactive oxygen species in bovine oocyte maturation in vitro
(Csiro Publishing, 2009-12)
The role of reactive oxygen species (ROS) in the in vitro maturation (IVM) of oocytes remains controversial. The aim of the present study was to determine possible fluctuations in ROS production during bovine oocyte IVM ...
Prematuration of bovine oocytes with butyrolactone I reversibly arrests meiosis without increasing meiotic abnormalities after in vitro maturation
(ELSEVIER IRELAND LTD, 2009)
Objectives: Asynchrony between nuclear and cytoplasmic maturation, and possibly damage to the oocyte meiotic spindle, limits the application of in vitro maturation (IVM) in assisted reproduction. Several studies have ...
Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide
(Elsevier B.V., 2011-02-01)
Ooplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ...
Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments
(Cambridge University Press, 2007-11-01)
In vitro-matured (IVM) bovine oocytes were activated with single and combined treatments of strontium (S), ionomycin (1) and 6-DMAP (D). Using oocytes IVM for 26 h, we observed that activation altered cell cycle kinetics ...
Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide
(Elsevier B.V., 2011-02-01)
Ooplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ...
Effect of kit ligand on natriuretic peptide precursor C and oocyte maturation in cattle
(Bioscientifica Ltd, 2016-11-01)
In vitro maturation (IVM) of oocytes in cattle is inefficient, and there is great interest in the development of approaches to improve maturation and fertilization rates. Intraovarian signalling molecules are being explored ...