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Quantitation of yeast cell-cell fusion using multicolor flow cytometry
(Wiley-liss, Div John Wiley & Sons Inc, 2015-09)
Mating of haploid Saccharomyces cerevisiae cells of opposite sex provides a powerful model system to study the cell-cell fusion. However, a rapid and standardized method is much needed for quantitative assessment of fusion ...
Aplicación de la proteína verde fluorescente en el monitoreo de cepas degradadoras de fenol
(Universidad Nacional de Colombia, 2001)
Diversos métodos se han descrito para monitorear microorganismos en muestras ambientales; recientemente se han diseñado sistemas para la marcación estable de cepas con genes testigos como los genes lux (luminiscencia) y ...
Beta-Actin shows limited mobility and is required only for supraphysiological insulin-stimulated glucose transport in young adult soleus muscle
(American Physiological Society, 2018)
Studies in skeletal muscle cell cultures suggest that the cortical actin cytoskeleton is a major requirement for insulin-stimulated glucose transport, implicating the beta-actin isoform, which in many cell types is the ...
Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches
(F1000Research, 2014-05)
Abstract Shiga toxin (Stx) is considered the main virulence factor in Shiga toxin-producing Escherichia coli (STEC) infections. Previously we reported the expression of biologically active Stx by eukaryotic cells in ...
Agrobacterium-mediated genetic transformation of Prunus salicina
(SPRINGER, 2008-08)
We report Agrobacterium tumefaciens-mediated
transformation of two Prunus salicina varieties,
‘Angeleno’ and ‘Larry Anne’, using a modification of
the hypocotyl slice technique previously described for
P. domestica. ...
Murine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryo
(Wiley-Blackwell, 2008-11-01)
We describe two replication incompetent retroviral vectors that co-express green fluorescent protein (GFP) and beta-galactosidase. These vectors incorporate either the avian reticuloendotheliosis (spleen necrosis virus; ...
Pharmacological Induction of Heme Oxygenase-1 Impairs Nuclear Accumulation of Herpes Simplex Virus Capsids upon Infection
(2017)
Heme oxygenase-1 (HO-1) is an inducible enzyme that is expressed in response to physical and chemical stresses, such as ultraviolet radiation, hyperthermia, hypoxia, reactive oxygen species (ROS), as well as cytokines, ...
La sobreexpresión del gen de enzina convertidora de angiotensina homóloga (ECA2) revierte la hipertensión arterial y el remodelado cardíaco experimental
(2010)
Objective Background. Over expression of the gene for homologous angiotensin I converting enzyme (ACE) is associated with prevention of angiotensin II dependent cardiac hypertrophy and fibrosis. Whether this over expression ...
MYB Transcription Factors and a Putative Flavonoid Transporter ABCC-Like are Differentially Expressed in Radiata Pine Seedlings Exposed to Inclination
(2018)
The response to inclination in plants is a well-studied biological process where hormones have been implicated in the modulation of vertical growth restoration. Studies on the involvement of auxins in differential growth ...
3D reconstruction of Artificially Structured Microbial Consortia (ASMC) by image analysis
(2009)
A biofilm is formed when microorganisms are attached to a surface and, within the whole biofilm; microorganisms are only a small volume fraction, which is complemented by the extracellular matrix of the structure. Inside ...