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ZapA, a possible virulence factor from Proteus mirabilis exhibits broad protease substrate specificity
(Associação Brasileira de Divulgação Científica, 2001-11-01)
The opportunistic bacterium Proteus mirabilis secretes a metalloprotease, ZapA, considered to be one of its virulence factors due to its IgA-degrading activity. However, the substrate specificity of this enzyme has not yet ...
Differences in substrate and inhibitor sequence specificity of human, mouse and rat tissue kallikreins
(Portland Press, 2004-06-15)
The kininogenase activities of mouse (mK1), rat (rK1) and human (hK1) tissue kallikrems were assayed with the bradykinin-containing synthetic peptides NH2-MTEMARRPPGFSPFRSVTVQ-NH2 (where Abz stands for o-aminobenzoyl) and ...
A novel activating effect of the regulatory subunit of protein kinase A on catalytic subunit activity
(Elsevier Science Inc, 2008-12)
The strength of the interaction between the catalytic and regulatory subunits in protein kinase A differs among species. The linker region from regulatory subunits is non-conserved. To evaluate the participation of this ...
Peptide synthesis: chemical or enzymatic
(Pontificia Universidad Católica de Valparaíso, 2007)
Investigation of Thrombin Activity with PAR 1-based Fluorogenic Peptides
(Bentham Science Publ Ltd, 2013-10-01)
Thrombin, a highly specific protease of blood coagulation, has two exosites that modulate its specificity. We designed two sets of synthetic substrate FRET peptides with 25- or 11-amino acids (aa) each, based on the PAR 1 ...
Expression and substrate specificity of a recombinant cysteine proteinase B of Leishmania braziliensis
(Elsevier B.V., 2008-10-01)
The cysteine proteinase B of Leishmania parasites is an important virulence factor. in this study we have expressed, isolated and characterized for the first time a recombinant CPB from Leishmania braziliensis, the causative ...
Positional-scanning combinatorial libraries of fluorescence resonance energy transfer peptides for defining substrate specificity of the angiotensin I-converting enzyme and development of selective C-domain substrates
(Amer Chemical Soc, 2004-12-21)
Positional-scanning combinatorial libraries of fluorescence resonance energy transfer peptides were used for the analyses of the S-3 to S-1' subsites of the somatic angiotensin I-converting enzyme (ACE). Substrate specificity ...
Analysis of peptides in prohormone convertase 1/3 null mouse brain using quantitative peptidomics
(WILEY-BLACKWELL, 2010)
P>Neuropeptides are produced from larger precursors by limited proteolysis, first by endopeptidases and then by carboxypeptidases. Major endopeptidases required for these cleavages include prohormone convertase (PC) 1/3 ...
IAF, QGF, and QDF peptides exhibit cholesterol-lowering activity through a statin-like HMG-CoA reductase regulation mechanism: In silico and in vitro approach
(2021-10-01)
In this study, in silico approaches are employed to investigate the binding mechanism of peptides derived from cowpea β-vignin and HMG-CoA reductase. With the obtained information, we designed synthetic peptides to evaluate ...