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Expression and substrate specificity of a recombinant cysteine proteinase B of Leishmania braziliensis
(Elsevier B.V., 2008-10-01)
The cysteine proteinase B of Leishmania parasites is an important virulence factor. in this study we have expressed, isolated and characterized for the first time a recombinant CPB from Leishmania braziliensis, the causative ...
Development of an operational substrate for ZapA, a metalloprotease secreted by the bacterium Proteus mirabilis
(Associação Brasileira de Divulgação Científica (ABRADIC), 2000-07-01)
The protease ZapA, secreted by Proteus mirabilis, has been considered to be a virulence factor of this opportunistic bacterium. The control of its expression requires the use of an appropriate methodology, which until now ...
A SELECTIVE ASSAY for ENDOOLIGOPEPTIDASE A BASED ON the CLEAVAGE of FLUOROGENIC SUBSTRATE STRUCTURALLY RELATED TO ENKEPHALIN
(Academic Press Inc Jnl-comp Subscriptions, 1990-12-14)
Chromogenic and fluorogenic glycosylated and acetylglycosylated peptides as substrates for serine, thiol and aspartyl proteases
(Munksgaard Int Publ Ltd, 1999-02-01)
We synthesized short chromogenic peptidyl-Arg-p-nitroanilides containing either (Gal beta)Ser or (Glc alpha,beta)Tyr at P-2 or P-3 sites as well as O-acetylated sugar moieties and studied their hydrolysis by bovine trypsin, ...
Discriminating between the activities of human neutrophil elastase and proteinase 3 using serpin-derived fluorogenic substrates
(Amer Soc Biochemistry Molecular Biology Inc, 2002-10-18)
Human neutrophil elastase (HNE) has long been linked to the pathology of a variety of inflammatory diseases and therefore is a potential target for therapeutic intervention. At least two other serine proteases, proteinase ...
Characterization of the substrate specificity of the major cysteine protease (cruzipain) from Trypanosoma cruzi using a portion-mixing combinatorial library and fluorogenic peptides
(Portland Press, 1997-04-15)
The substrate specificity of the major cysteinyl proteinase of the parasitic protozoan Trypanosoma cruzi (cruzipain) was investigated, by combinatorial replacement of amino acid residues at positions P-5-P'(5), using a ...
Specificity of prohormone convertase 2 on proenkephalin and proenkephalin-related substrates
(Amer Soc Biochemistry Molecular Biology Inc, 1998-08-28)
In the central and peripheral nervous systems, the neuropeptide precursor proenkephalin must be endoproteolytically cleaved by enzymes known as prohormone convertases 1 and 2 (PC1 and PC2) to generate opioid-active ...
Development of an operational substrate for ZapA, a metalloprotease secreted by the bacterium Proteus mirabilis
(Associação Brasileira de Divulgação Científica (ABRADIC), 2014)
Synthesis and hydrolysis by cathepsin B of fluorogenic substrates with the general structure benzoyl-X-ARG-MCA containing non-natural basic amino acids at position X
(Elsevier B.V., 2001-05-05)
We synthesized one series of fluorogenic substrates for cathepsin B derived from the peptide Bz-F-R-MCA (Bz = benzoyl, MCA = 7-methyl-coumarin amide) substituting Phe at the P(2) position by non-natural basic amino acids ...
Synthesis and hydrolysis by cysteine and serine proteases of short internally quenched fluorogenic peptides
(Academic Press Inc, 2001-06-01)
We developed sensitive substrates for cysteine proteases and specific substrates for serine proteases based on short internally quenched fluorescent peptides, Abz-F-R-X-EDDnp, where Abz (ortho-aminobenzoic acid) is the ...