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On the efficient bio-incorporation of 5-hydroxy-tryptophan in recombinant proteins expressed in Escherichia coli with T7 RNA polymerase-based vectors
(Academic Press Inc Elsevier Science, 2017)
Biosynthetic incorporation of non-canonic amino acids is an attractive strategy to introduce new properties in recombinant proteins. Trp analogs can be incorporated in recombinant proteins replacing regular Trp during ...
Fluorescence Quantum Yields in Complex Environments from QM-MM TDDFT Simulations: The Case of Indole in Different Solvents
(American Chemical Society, 2020-11)
Fluorescence is commonly exploited to probe microscopic properties. An important example is tryptophan in protein environments, where variations in fluorescence quantum yield, and in absorption and emission maxima, are ...
Development of chemically defined media to express trp-analog-labeled proteins in a lactococcus lactis trp auxotroph
(Pontificia Universidade Catolica Campinas, 2016)
Chemically defined media for growth of Lactococcus lactis strains contain about 50 components, making them laborious and expensive growth media. However, they are crucial for metabolism studies as well as for expression ...
The Violacein Biosynthesis Monitored by Multi-Wavelength Fluorescence Spectroscopy and by the PARAFAC Method
(Soc Brasileira QuimicaSao PauloBrasil, 2012)
Fluorescence studies of ATP-diphosphohydrolase from Solanum tuberosum var. Desiree
(PERGAMON-ELSEVIER SCIENCE LTD, 2000-08)
Chemical modification of potato apyrase suggests that tryptophan residues are close to the nucleotide binding site. K-d values (+/- Ca2+) for the complexes of apyrase with the non-hydrolysable phosphonate adenine nucleotide ...
Could antibodies be stable and effective after years of storage?
(Fiocruz/Instituto de Tecnologia em Imunobiológicos, 2022)
Avaliação da estabilidade de IgG de cabra anti-humano marcado com FITC por espectroscopia de fluorescência intrínsica do triptofano (W)
(Fiocruz/Instituto de Tecnologia em Imunobiológicos, 2018)
Exploring in vivo violacein biosynthesis by application of multivariate curve resolution on fused UV-VIS absorption, fluorescence, and liquid chromatography-mass spectrometry data
(Springer HeidelbergHeidelbergAlemanha, 2013)
Apolipoprotein A-I configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process
(Elsevier, 2013-11-05)
Discoidal high-density lipoproteins (D-HDL) are critical intermediates in reverse cholesterol transport. Most of the present knowledge of D-HDL is based on studies with reconstituted lipoprotein complexes of apolipoprotein ...
Interaction of a synthetic antimicrobial peptide with model membrane by fluorescence spectroscopy
(Springer, 2013-12-01)
Static and time-resolved fluorescence of tryptophan and ortho-aminobenzoic acid was used to investigate the interaction of the synthetic antimicrobial peptide L1A (IDGLKAIWKKVADLLKNT-NH2) with POPC and POPC:POPG. N-acetylated ...