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Low temperature induced changes in activity and protein levels of the enzymes associated to conversion of starch to sucrose in banana fruit
(ELSEVIER SCIENCE BV, 2011)
Storage at low temperature is the most frequently used method to extend the shelf life of banana fruit, and is fundamental for extended storage and transport over long distances. However, storage and transport conditions ...
Putative pyrophosphate phosphofructose 1-kinase genes identified in sugarcane may be getting energy from pyrophosphate
(2003-12-01)
Pyrophosphate-dependent phosphofructokinase (PPi-PFK) has been detected in several types of plant cells, but the gene has not been reported in sugar cane. Using Citrux paradixi PPi-PFK gene (AF095520 and AF095521) sequences ...
Putative pyrophosphate phosphofructose 1-kinase genes identified in sugarcane may be getting energy from pyrophosphate
(2003-12-01)
Pyrophosphate-dependent phosphofructokinase (PPi-PFK) has been detected in several types of plant cells, but the gene has not been reported in sugar cane. Using Citrux paradixi PPi-PFK gene (AF095520 and AF095521) sequences ...
Acid invertase as a serious candidate to control the balance sucrose versus (glucose plus fructose) of banana fruit during ripening
(Elsevier B.V., 2011-06-10)
Six banana varieties: 3 'dessert' ones: 'IDN 110'; 'Kirun'; and 'Grande Naine', and 3 'cooking' ones: 'Galeo'; 'Sowmuk'; and 'French' were used to investigate the relationship between sugar profiles and activities of ...
Relaciones De Estructura A Función Y Evolución De Enzimas Del Metabolismo Del Carbono. Caracterización De Enzimas Del Metabolismo De Hidratos De Carbono En Bacterias Autotróficas Y Heterotróficas
(2012-12-26)
Esta tesis profundiza el estudio comparativo del metabolismo de los hidratos de carbono entre organismos heterótrofos y autótrofos. Para tal caso, se abordó el estudio in vitro de las enzimas que conforman las rutas ...
Influence of Hot Air and Modified Atmosphere Packaging on Chilling Injury Incidence and Metabolism of Soluble Sugars in Eggplant Fruit (Solanum melongena L.)
(Nextgen Research Publication, 2017-11)
In this study, the influence of hot air (HA) and passive modified atmosphere packaging (MAP) on chilling injury (CI) and the metabolism of soluble carbohydrates on eggplant fruits stored at 3 °C for 9 days were investigated. ...
Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate
(American Society of Plant Biologist, 2013-11)
Many plants accumulate substantial starch reserves in their leaves during the day and remobilize them at night to provide carbon and energy for maintenance and growth. In this paper, we explore the role of a sugar-signaling metabolite, trehalose-6-phosphate (Tre6P), in regulating the accumulation and turnover of transitory starch in Arabidopsis (Arabidopsis thaliana) leaves. Ethanol-induced overexpression of trehalose-phosphate synthase during the day increased Tre6P levels up to 11-fold. There was a transient increase in the rate of starch accumulation in the middle of the day, but this was not linked to reductive activation of ADP-glucose pyrophosphorylase. A 2- to 3-fold increase in Tre6P during the night led to significant inhibition of starch degradation. Maltose and maltotriose did not accumulate, suggesting that Tre6P affects an early step in the pathway of starch degradation in the chloroplasts. Starch granules isolated from induced plants had a higher orthophosphate content than granules from noninduced control plants, consistent either with disruption of the phosphorylation-dephosphorylation cycle that is essential for efficient starch breakdown or with inhibition of starch hydrolysis by beta-amylase. Nonaqueous fractionation of leaves showed that Tre6P is predominantly located in the cytosol, with estimated in vivo Tre6P concentrations of 4 to 7 µM in the cytosol, 0.2 to 0.5 µM in the chloroplasts, and 0.05 µM in the vacuole. It is proposed that Tre6P is a component in a signaling pathway that mediates the feedback regulation of starch breakdown by sucrose, potentially linking starch turnover to demand for sucrose by growing sink organs at night....