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Identification of two putative reference genes from grapevine suitable for gene expression analysis in berry and related tissues derived from RNA-Seq data
(BMC, 2013-12)
Background: Data normalization is a key step in gene expression analysis by qPCR. Endogenous control genes are used to estimate variations and experimental errors occurring during sample preparation and expression measurements. ...
Selection of suitable reference genes for gene expression studies in myxosporean (Myxozoa, Cnidaria) parasites
(Nature Publishing Group, 2019-12)
Myxozoans (Cnidaria: Myxozoa) are an extremely diversified group of endoparasites some of which are causative agents of serious diseases in fish. New methods involving gene expression studies have emerged over the last ...
Selection and validation of reference genes for functional studies in the Calliphoridae family
(Milwaukee, 2014)
The genera Cochliomyia and Chrysomya contain both obligate and saprophagous flies, which allows the comparison of different feeding habits between closely related species. Among the different strategies for comparing these ...
Transcriptome-based identification and validation of reference genes for plant-bacteria interaction studies using Nicotiana benthamiana
(Nature Publishing Group, 2019-02-07)
RT-qPCR is a widely used technique for the analysis of gene expression. Accurate estimation of transcript abundance relies strongly on a normalization that requires the use of reference genes that are stably expressed in ...
A new set of reference genes for RT-qPCR assays in the yeast Dekkera bruxellensis
(Canadian Science Publishing, Nrc Research PressOttawaCanadá, 2012)
Identification and validation of quantitative real-time reverse transcription PCR reference genes for gene expression analysis in teak (Tectona grandis L.f.)
(2014-07-22)
Abstract
Background
Teak (Tectona grandis L.f.) is currently the preferred choice of the timber trade for fabrication of woody products due to its extraordinary qualities and ...
Suitable reference genes for RT-qPCR analysis in Dichelops melacanthus (Hemiptera: Pentatomidae)
(2020-07-01)
The relative quantification of gene expression is mainly realized through reverse transcription-quantitative PCR (RT-qPCR). However, the accuracy of this technique is deeply influenced by the expression stability of the ...
Reference gene selection for gene expression analysis of oocytes collected from dairy cattle and buffaloes during winter and summer
(PLOSSão Francisco, 2014-03)
Oocytes from dairy cattle and buffaloes have severely compromised developmental competence during summer. While analysis of gene expression is a powerful technique for understanding the factors affecting developmental ...
Reference Gene Selection for Gene Expression Analysis of Oocytes Collected from Dairy Cattle and Buffaloes during Winter and Summer
(Public Library Science, 2014-03-27)
Oocytes from dairy cattle and buffaloes have severely compromised developmental competence during summer. While analysis of gene expression is a powerful technique for understanding the factors affecting developmental ...