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Extraction of recombinant proteins from Escherichia coli by cell disruption with aqueous solutions of surface-active compounds
(2018-07-01)
BACKGROUND: Green fluorescent protein (GFP) is used extensively as a biomarker due to its unique spectral and fluorescence characteristics. GFP is usually obtained from recombinant strains of Escherichia coli (E. coli) ...
Desenvolvimento de plataformas sustentáveis para estabilização e purificação da Proteína Verde Fluorescente recombinante produzida por Escherichia coli
(Universidade Estadual Paulista (Unesp), 2020-11-26)
A biotecnologia permitiu o desenvolvimento de novas biomoléculas para aplicações médicas e industriais. No entanto, o uso de muitos produtos biológicos notáveis ainda é limitado em grande escala devido às suas baixas ...
Cellulose-Builder: A toolkit for building crystalline structures of cellulose
(Wiley-blackwellMaldenEUA, 2012)
Evaluation of Solid-Phase Microextraction using a Polythiophene Film and Liquid Chromatography with Spectrophotometric Detection for the Determination of Antidepressants in Plasma Samples
(SOC BRASILEIRA QUIMICASAO PAULO, 2012)
Polythiophene (PTh) phase electropolymerized on the stainless steel wire was evaluated as solid-phase microextraction (SPME), and analysis by liquid chromatography with spectrophotometric detection (LC-UV) for determination ...
Evaluation of solid-phase microextraction using a polythiophene film and liquid chromatography with spectrophotometric detection for the determination of antidepressants in plasma samples
(Sociedade Brasileira de Química, 2012)
Polythiophene (PTh) phase electropolymerized on the stainless steel wire was evaluated as solid-phase microextraction (SPME), and analysis by liquid chromatography with spectrophotometric detection (LC-UV) for determination ...
Líquidos iônicos como extratores verdes para obtenção de carotenoides da casca de laranja
(Universidade Federal de São Paulo (UNIFESP), 2019-12-04)
The increased concern about environmental and sustainability issues has encouraged the search for alternative strategies in several productive fields, in order to minimize the environment impacts and the risks to living ...
NorHarmane containing ionic liquid matrices for low molecular weight MALDI-MS carbohydrate analysis: The perfect couple with α-cyano-4-hydroxycinnamic acid
(John Wiley & Sons Ltd, 2019-05)
Cinnamic acid derivatives, particularly α-cyano-4-hydroxycinnamic acid (E-α-cyano-4-hydroxycinnamic acid or (E)-2-cyano-3-(4-hydroxyphenyl)prop-2-enoate; CHCA), have been extensively used especially for protein and peptide ...
Uma análise bibliométrica sobre os líquidos iônicos na engenharia química: aplicação da metodologia ProKnow-C em conjunto com software VosViewer
(Universidade Tecnológica Federal do ParanáFrancisco BeltraoBrasilEngenharia QuímicaUTFPR, 2022-06-24)
The study of ionic liquids has become increasingly growing and vast, covering several areas of application due to their particularities. Aiming to find possible areas of application in Chemical Engineering, a bibliometric ...
Extração e purificação do biofármaco antileucêmico L-asparaginase (ASNase) utilizando sistemas aquosos bifásicos com polímeros e líquidos iônicos
(Universidade Estadual Paulista (Unesp), 2019-03-22)
A L-asparaginase destaca-se como um importante biofármaco utilizado no tratamento de leucemia, além da sua utilização na indústria alimentícia. Seu alto custo de produção se deve principalmente às etapas de purificação, ...
Curvophilic-curvophobic balance of monoalkyl-mannosides determines the magnitude of disturbance promoted in synthetic bilayers
(Elsevier B.V., 2019)
The principles governing surfactant-lipid interactions, involved in biological membrane solubilization and reconstitution processes, are a topic of great interest in the field of membrane biochemistry and biophysics. Among ...