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Development and comparative analysis of yeast protein extraction protocols for mass spectrometry
(2019-02-15)
Mass spectrometry is the most used method for protein identification and quantification. Here we developed four protein extraction protocols precisely for mass spectrometry, and we compared with other ones already published. ...
Liver iron modulates hepcidin expression during chronically elevated erythropoiesis in mice
(Wiley, 2013)
The liver-derived peptide hepcidin controls the balance between iron demand and iron supply. By inhibiting the iron export activity of ferroportin, hepcidin modulates iron absorption and delivery from the body's stores. ...
Detection of AA-type amyloid protein in labial salivary glands
(Medicina Oral, 2011)
Objectives: Among the diverse forms of amyloidosis, secondary type is the most frequent one. Diagnosis of amyloid deposition is based on the identification of the fibrillary protein amyloid by means of Congo Red (CR) or ...
An alternative easy method for antibody purification and analysis of protein-protein interaction-using GST fusion proteins immobilized onto glutathione-agarose
(Springer, 2014-01)
Immobilization of small proteins designed to perform protein-protein assays can be a difficult task. Often, the modification of reactive residues necessary for the interaction between the immobilized protein and the matrix ...
Chasing coevolutionary signals in intrinsically disordered proteins complexes
(Nature, 2020-10)
Intrinsically disordered proteins/regions (IDPs/IDRs) are crucial components of the cell, they are highly abundant and participate ubiquitously in a wide range of biological functions, such as regulatory processes and cell ...