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An integrated map of HIV genome-wide variation from a population perspective
Background: The HIV pandemic is characterized by extensive genetic variability, which has challenged the development of HIV drugs and vaccines. Although HIV genomes have been classified into different types, groups, subtypes ...
On the role of frustration in the energy landscapes of allosteric proteins
(National Academy of Sciences, 2011-03)
Natural protein domains must be sufficiently stable to fold but often need to be locally unstable to function. Overall, strong energetic conflicts are minimized in native states satisfying the principle of minimal frustration. ...
Biochemical and biophysical characterization of the RVB-1/RVB-2 protein complex, the RuvBL/RVB homologues in Neurospora crassa
(2021-12-01)
The RVB proteins, composed of the conservative paralogs, RVB1 and RVB2, belong to the AAA+ (ATPases Associated with various cellular Activities) protein superfamily and are present in archaea and eukaryotes. The most ...
Expression of the multimeric and highly immunogenic brucella spp. Lumazine synthase fused to bovine rotavirus VP8D as a scaffold for antigen production in tobacco chloroplasts
(Frontiers Media S.A., 2015-12)
Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein which can accommodate foreign polypeptides or protein domains fused to its N-termini, markedly increasing their immunogenicity. The inner ...
Double autoinhibition mechanism of signal transduction ATPases with numerous domains (STAND) with a tetratricopeptide repeat sensor
(Oxford University Press, 2019-04)
Upon triggering by their inducer, signal transduction ATPases with numerous domains (STANDs), initially in monomeric resting forms, multimerize into large hubs that activate target macromolecules. This process requires ...
Production of a Highly Immunogenic Antigen from SARS-CoV-2 by Covalent Coupling of the Receptor Binding Domain of Spike Protein to a Multimeric Carrier
(Cold Spring Harbor Laboratory Press, 2021-04-26)
Since the discovery of SARS-CoV-2, several antigens have been proposed to be part of COVID-19 vaccines. The receptor binding domain (RBD) of Spike protein is one of the promising candidates to develop effective vaccines ...
Functional Sites Induce Long-Range Evolutionary Constraints in Enzymes
(Public Library of Science, 2016-05)
Functional residues in proteins tend to be highly conserved over evolutionary time. However, to what extent functional sites impose evolutionary constraints on nearby or even more distant residues is not known. Here, we ...
Identification of a membrane-bound prepore species clarifies the lytic mechanism of actinoporins
(American Society for Biochemistry and Molecular Biology, 2016-09)
Pore-forming toxins (PFTs) are cytolytic proteins belonging to the molecular warfare apparatus of living organisms. The assembly of the functional transmembrane pore requires several intermediate steps ranging from a ...
Chemical Modification of Lysozyme, Glucose 6‑Phosphate Dehydrogenase, and Bovine Eye Lens Proteins Induced by Peroxyl Radicals: Role of Oxidizable Amino Acid Residues
(2013)
Chemical and structural alterations to lysozyme
(LYSO), glucose 6-phosphate dehydrogenase (G6PD), and
bovine eye lens proteins (BLP) promoted by peroxyl radicals
generated by the thermal decomposition of 2,2′-azobis(2 ...