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Novel intein-based self-cleaving affinity tag for recombinant protein production in Escherichia coli
(Elsevier Science, 2021-05)
We evaluated several intein-based self-cleaving affinity tags for expression and single-step affinity chromatography purification of recombinant proteins produced in Escherichia coli. We used human growth hormone (hGH) as ...
A dimerization interface mediated by functionally critical residues creates interfacial disulfide bonds and copper sites in CueP
(Elsevier, 2014-08)
CueP confers bacterial copper resistance in the periplasm, particularly under anaerobic conditions, through an unknown mechanism. The only available structure and limited solution data suggest that CueP forms noncovalent ...
Structural basis for c-di-GMP-mediated inside-out signaling controlling periplasmic proteolysis
(Public Library Science - PLOSSan Francisco, 2011-02)
The bacterial second messenger bis-(3′–5′) cyclic dimeric guanosine monophosphate (c-di-GMP) has emerged as a central regulator for biofilm formation. Increased cellular c-di-GMP levels lead to stable cell attachment, which ...
Clonagem, expressão heteróloga e caracterização parcial da trealase periplasmática de Xanthomonas citri subsp. citri e do seu envolvimento com a fitopatogenicidade
(Universidade Federal de São CarlosUFSCarPrograma de Pós-Graduação em Genética Evolutiva e Biologia Molecular - PPGGEvCâmpus São Carlos, 2015-03-03)
Citrus canker imposes damages to citriculture by causing drop in productivity and fruit
quality and the absence of effective control and cure. Thus, the economic potential of citrus is limited in part by this disease ...
The crystal structure of the leptospiral hypothetical protein LIC12922 reveals homology with the periplasmic chaperone SurA
(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2011)
Leptospirosis is a world spread zoonosis caused by members of the genus Leptospira. Although leptospires were identified as the causal agent of leptospirosis almost 100 years ago, little is known about their biology, which ...
Cytoplasmic copz-like protein and periplasmic rusticyanin and acop proteins as possible copper resistance determinants in acidithiobacillus ferrooxidans atcc 23270
(2016)
Acidophilic organisms, such as Acidithiobacillus ferrooxidans, possess high-level resistance to copper and other metals. A. ferrooxidans contains canonical copper resistance determinants present in other bacteria, such as ...