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Hydrophobic interaction chromatography for purification of monoPEGylated RNase A
(ELSEVIER SCIENCE BV, 2012-06-15)
The chromatographic methods used for the purification of PEGylated proteins are mainly Size Exclusion
(SEC) and Ion Exchange Chromatography (IEX). Although the PEGylation affects the protein hydrophobicity,
Hydrophobic ...
Non-chromatographic determination of ultratraces of V(V) and V(IV) based on a double column solid phase extraction flow injection system coupled to electrothermal atomic absorption spectrometry
(Elsevier Science, 2009-08)
In this work, a non-chromatographic procedure for the on-line determination of ultratraces of V(V) and V(IV) is presented. The method involves a solid phase extraction-flow injection system coupled to electrothermal atomic ...
Recuperação de sílica gel utilizando tratamento oxidativo
(Universidade Tecnológica Federal do ParanáPonta GrossaBrasilDepartamento Acadêmico de Engenharia QuímicaEngenharia QuímicaUTFPR, 2021-12-03)
Silica gel is a synthetic amorphous silica widely used in laboratory techniques for separating components from substances. It is considered a highly adsorbent, non-toxic, chemically inert and stable compound; characteristics ...
Experimental validation of the predictions of a mathematical model for protein purification and tag selection
(2010)
To improve downstream processing, protein engineering can be used to modify particular properties of a protein, such as specific affinity, charge, or hydrophobicity. The most common modification involves the peptide tags ...
Evaluación diagnóstica de fracciones cromatográficas de Fasciola hepatica mediante Western Blot y ELISA en animales infectados
(UNIVERSIDAD AUSTRAL CHILE, FACULTAD CIENCIAS VETERINARIAS, 1997)
The antigenic components of excretory-secretory products of adult F. hepatica, were separated by gel filtration chromatography (Sephacryl S-300) and then analized by polyacrylamide gel electrophoresis (SDS-PAGE), followed ...
Cytotoxic non-aromatic B-ring flavanones from Piper carniconnectivum C. DC.
(Elsevier B.V., 2014-01-01)
The EtOAc extract from the leaves of Piper carniconnectivum C. DC. was subjected to chromatographic separation to afford two non-aromatic B-ring flavanone compounds: 5-hydroxy-2-(1'-hydroxy-4'-oxo-cyclohex-2'-en-1'-yl)-6 ...
Simultaneous LC/MS analysis of carotenoids and fat-soluble vitamins in Costa Rican avocados (Persea americana Mill.)
(2019)
Avocado (a fruit that represents a billion-dollar industry) has become a relevant crop in global trade. The benefits of eating avocados have also been thoroughly described as they contain important nutrients needed to ...