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Biosynthesis of an antiviral compound using a stabilized phosphopentomutase by multipoint covalent immobilization
(Elsevier Science, 2017-03)
Ribavirin is a synthetic guanosine analogue with a broad‐spectrum of antiviral activity. It is clinically <br />effective against several viruses, such as respiratory syncytial virus, several hemorrhagic fever viruses and ...
A highly stable biocatalyst obtained from covalent immobilization of a non-commercial cysteine phytoprotease
(OMICS, 2015-05)
In this work, araujiain (enzymatic preparation obtained from the latex of Araujia hortorum fruits) was successfully immobilized on glyoxyl-agarose via multipoint covalent attachment. Thus, good efficiency of immobilization ...
Descoloração de azul de bromofenol utilizando peroxidase imobilizada em pó de sabugo de milho altamente ativado
(2013-11-19)
The aim of the present study was to evaluate the efficacy of peroxidase immobilized on corncob powder for the discoloration of dye. Peroxidase was extracted from soybean seed coat, followed by amination of the surface of ...
Stabilization by multipoint covalent attachment of a biocatalyst with polygalacturonase activity used for juice clarification
(Elsevier, 2016-03)
Derivatized-agarose supports are suitable for enzyme immobilization by different methods, taking advantage of different physical, chemical and biological conditions of the protein and the support. In this study, agarose ...
Protein Hydrolysis by Immobilized and Stabilized Trypsin
(Wiley-Blackwell, 2011-05-01)
The preparation of novel immobilized and stabilized derivatives of trypsin is reported here. The new derivatives preserved 80% of the initial catalytic activity toward synthetic substrates [benzoyl-arginine p-nitroanilide ...
Protein Hydrolysis by Immobilized and Stabilized Trypsin
(Wiley-Blackwell, 2011-05-01)
The preparation of novel immobilized and stabilized derivatives of trypsin is reported here. The new derivatives preserved 80% of the initial catalytic activity toward synthetic substrates [benzoyl-arginine p-nitroanilide ...
Covalent immobilization of Candida rugosa lipase at alkaline pH and their application in the regioselective deprotection of per-O-acetylated thymidine
(MDPI AG, 2016-08)
Lipase from Candida rugosa (CRL) was stabilized at alkaline pH to overcome the inactivation problem and was immobilized for the first time by multipoint covalent attachment on different aldehyde-activated matrices. PEG was ...
Protein Hydrolysis by Immobilized and Stabilized Trypsin
(Wiley-Blackwell, 2014)