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Human recombinant membrane-bound aminopeptidase P: production of a soluble form and characterization using novel, internally quenched fluorescent substrates
(Portland Press Ltd, 2005-01-15)
APP (aminopeptidase P) has the unique ability to cleave the N-terminal amino acid residue from peptides exhibiting a proline at P-1'. Despite its putative involvement in the processing of bioactive peptides, among them the ...
Binding of Sulpiride to Seric Albumins
(MDPI, 2017)
Interaction of adrenocorticotropin peptides with microheterogeneous systems - A fluorescence study
(ELSEVIER SCIENCE BV, 2009)
Adrenocorticotropin (ACM) and alpha-melanocyte stimulating hormone (alpha-MSH) are peptides which present many physiological effects related to pigmentation, motor and sexual behavior, learning and memory, analgesia, ...
Detection of the basement membrane-degrading proteolytic activity of Paracoccidioides brasiliensis after SDS-PAGE using agarose overlays containing Abz-MKALTLQ-EDDnp
(Associação Brasileira de Divulgação Científica, 1999-05-01)
We have characterized, in the Paracoccidioides brasiliensis yeast phase, an exocellular SH-dependent serine proteinase activity against Abz-MKRLTL-EDDnp and analogous fluorescent-quenched peptides, and showed that it is ...
Development of an operational substrate for ZapA, a metalloprotease secreted by the bacterium Proteus mirabilis
(Associação Brasileira de Divulgação Científica, 2000-07-01)
The protease ZapA, secreted by Proteus mirabilis, has been considered to be a virulence factor of this opportunistic bacterium. The control of its expression requires the use of an appropriate methodology, which until now ...
Development of an operational substrate for ZapA, a metalloprotease secreted by the bacterium Proteus mirabilis
(Associação Brasileira de Divulgação Científica (ABRADIC), 2000-07-01)
The protease ZapA, secreted by Proteus mirabilis, has been considered to be a virulence factor of this opportunistic bacterium. The control of its expression requires the use of an appropriate methodology, which until now ...
Differences in substrate and inhibitor sequence specificity of human, mouse and rat tissue kallikreins
(Portland Press, 2004-06-15)
The kininogenase activities of mouse (mK1), rat (rK1) and human (hK1) tissue kallikrems were assayed with the bradykinin-containing synthetic peptides NH2-MTEMARRPPGFSPFRSVTVQ-NH2 (where Abz stands for o-aminobenzoyl) and ...
N-terminal acetylation of a mastoparan-like peptide enhances PE/PG segregation in model membranes
(2020-10-01)
The synthetic peptides L1A and its acetylated analog (acL1A) display potent Gram-negative bactericidal activities without being hemolytic. We have gathered evidence that the N-terminal acetylation of L1A enhances the lytic ...
Synthesis and hydrolysis by cysteine and serine proteases of short internally quenched fluorogenic peptides
(Academic Press Inc, 2001-06-01)
We developed sensitive substrates for cysteine proteases and specific substrates for serine proteases based on short internally quenched fluorescent peptides, Abz-F-R-X-EDDnp, where Abz (ortho-aminobenzoic acid) is the ...
Conformational basis for the biological activity of TOAC-labeled angiotensin II and bradykinin: Electron paramagnetic resonance, circular dichroism, and fluorescence studies
(2004-08-05)
N-Terminally and internally labeled analogues of the hormones angiotensin (AII, DRVYIHPF) and bradykinin (BK, RPPGFSPFR) were synthesized containing the paramagnetic amino acid 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4- ...