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A simple solution for antibody signal enhancement in immunofluorescence and triple immunogold assays
(2016)
Immunolocalization techniques are standard in biomedical research. Tissue fixation with aldehydes and cell membrane permeabilization with detergents can distort the specific binding of antibodies to their high affinity ...
Distribution of epitopes of Trypanosoma cruzi amastigotes during the intracellular life cycle within mammalian cells
(Soc Protozoologists, 1997-07-01)
In this study we have examined the distribution of epitopes defined by monoclonal antibodies raised against Trypanosoma cruzi amastigotes during the intracellular life cycle of the parasite. We have raised monoclonal ...
Actin-rich structures formed during the invasion of cultured cells by infective forms of Trypanosoma cruzi
(Urban & Fischer Verlag, 1999-12-01)
Previous work has shown that Trypanosoma cruzi extracellular amastigotes as well as metacyclic trypomastigotes infect cultured cells in a highly specific parasite form-cell type interaction. In this work we have investigated ...
Target cells for antibodies detection in rabies vaccine control
(Elsevier, 2014-03)
Rabies causes one of the most lethal zoonotic diseases, with more than 55,000 deaths reported annually. Prevention is based on pre‑exposure vaccination of individuals at high risk of contracting rabies, and mass vaccination ...
A Smartphone-Based Low-Cost Inverted Laser Fluorescence Microscope for Disease Diagnosis
(MDPI, 2022)
Fluorescence microscopy is an important tool for disease diagnosis, often requiring costly optical components, such as fluorescence filter cubes and high-power light sources. Due to its high cost, conventional fluorescence ...