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Thermodynamics of DNA with hump Morse potential
(2016-01-01)
The thermal denaturation of DNA, i.e. the separation of the two strands is a phenomenon caused by the amplitude of the vibrations of the bases, therefore it is necessary to know how such separation is generated in order ...
Production and biochemical characterization of xylanases synthesized by the thermophilic fungus Rasamsonia emersonii S10 by solid-state cultivation
(2021-04-01)
The xylanolytic enzyme complex hydrolyzes xylan, and these enzymes have various industrial applications. The goal of this work was to characterize the endoxylanases produced by the thermophilic fungus Rasamsonia emersonii ...
Desenvolvimento de uma representação mesoscópica para marcadores fluorescentes em DNA e predição de intensidade de fluorescência
(Universidade Federal de Minas GeraisBrasilICB - INSTITUTO DE CIÊNCIAS BIOLOGICASPrograma de Pós-Graduação em BioinformaticaUFMG, 2020-02-18)
Fluorescent dyes attached to DNA molecules are used in biomedicine and biotechnology areas, for example, and they can be studied with mesoscopic models such as Peyrard-Bishop model (PB). In particular, Cy3 and Cy5 dyes ...
Isolamento, caracterização termodinâmica e estudos bioquímicos da lectina de sementes dormentes e de cotilédones de sementes germinadas da leguminosa Macrotyloma axillare
(Universidade Federal de Minas GeraisUFMG, 2008-09-19)
The Macrotyloma axillare seed lectin (LMA) is an N-acetyl-galactosamine (GalNac) specific lectin very important in biothecnology because the specific hemmaglutination by A1 human erythrocytes. In this work, the LMA from ...
Thermostable variants of the recombinant xylanase A from Bacillus subtilis produced by directed evolution show reduced heat capacity changes
(WILEY-LISS, 2008)
Directed evolution techniques have been used to improve the thermal stability of the xylanase A from Bacillus subtilis (XylA). Two generations of random mutant libraries generated by error prone PCR coupled with a single ...
On the temperature stability of extracellular hemoglobin of Glossoscolex paulistus, at different oxidation states: SAXS and DLS studies
(Elsevier B.V., 2012-04-01)
Glossoscolex paulistus hemoglobin (HbGp) was studied by dynamic light scattering (DLS) and small angle X-ray scattering (SAXS). DLS melting curves were measured for met-HbGp at different concentrations. SAXS temperature ...
On the temperature stability of extracellular hemoglobin of Glossoscolex paulistus, at different oxidation states: SAXS and DLS studies
(Elsevier B.V., 2012-04-01)
Glossoscolex paulistus hemoglobin (HbGp) was studied by dynamic light scattering (DLS) and small angle X-ray scattering (SAXS). DLS melting curves were measured for met-HbGp at different concentrations. SAXS temperature ...
Characterization of a Hexameric Exo-Acting GH51 alpha-l-Arabinofuranosidase from the Mesophilic Bacillus subtilis
(Humana Press Inc, 2013-11-01)
alpha-l-Arabinofuranosidases (alpha-l-Abfases, EC 3.2.1.55) display a broad specificity against distinct glycosyl moieties in branched hemicellulose and recent studies have demonstrated their synergistic use with cellulases ...