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Mostrando ítems 11-20 de 35
Chromogenic and fluorogenic glycosylated and acetylglycosylated peptides as substrates for serine, thiol and aspartyl proteases
(Munksgaard Int Publ Ltd, 1999-02-01)
We synthesized short chromogenic peptidyl-Arg-p-nitroanilides containing either (Gal beta)Ser or (Glc alpha,beta)Tyr at P-2 or P-3 sites as well as O-acetylated sugar moieties and studied their hydrolysis by bovine trypsin, ...
Subsite substrate specificity of midgut insect chymotrypsins
(Elsevier B.V., 2008-06-01)
Insect chymotrypsins are distinctively sensitive to plant protein inhibitors, suggesting that they differ in subsite architecture and hence in substrate specificities. Purified digestive chymotrypsins from insects of three ...
Hexose transporter glut1 harbors several distinct regulatory binding sites for flavones and tyrphostins
(AMER CHEMICAL SOC, 2011)
The facilitative hexose transporter GLUT1 activity is blocked by tyrosine kinase inhibitors that include natural products such as flavones and isoflavones and synthetic compounds such as tyrphostins, molecules that are ...
Propriedades hidrolíticas de cisteíno-peptidases recombinantes de Tripanossomatídeos
(Universidade Federal de São Paulo (UNIFESP), 2004)
Trypanosoma cruzi cysteine proteinase cruzipain contains a 130-aminoacid C-terminal extension, in addition to the catalytic domain. Natural cruzipain is a complex of isoforms, because of the simultaneous expression of ...
Expression and substrate specificity of a recombinant cysteine proteinase B of Leishmania braziliensis
(Elsevier B.V., 2008-10-01)
The cysteine proteinase B of Leishmania parasites is an important virulence factor. in this study we have expressed, isolated and characterized for the first time a recombinant CPB from Leishmania braziliensis, the causative ...
Comparison of the specificity, stability and individual rate constants with respective activation parameters for the peptidase activity of cruzipain and its recombinant form, cruzain, from Trypanosoma cruzi
(Blackwell Publishing Ltd, 2001-12-01)
The Trypanosoma cruzi cysteine protease cruzipain contains a 130-amino-acid C-terminal extension, in addition to the catalytic domain. Natural cruzipain is a complex of isoforms, because of the simultaneous expression of ...
Characterization of two cysteine proteinases secreted by Fasciola hepatica and demonstration of their kininogenase activity
(Elsevier B.V., 2001-09-03)
We have isolated and purified two cysteine proteinases of molecular weights 25 and 26 kDa, secreted by Fasciola hepatica adult worm. Their 15 N-terminal residues were found to be identical to those of earlier described ...
The emergence of new catalytic abilities in an endoxylanase from family GH10 by removing an intrinsically disordered region
(MDPI, 2022)
Endoxylanases belonging to family 10 of the glycoside hydrolases (GH10) are versatile in the use of different substrates. Thus, an understanding of the molecular mechanisms underlying substrate specificities could be very ...
Characterization of thiol-, aspartyl-, and thiol-metallo-peptidase activities in Madin-Darby canine kidney cells
(Wiley-Blackwell, 2000-01-01)
We combined fluorogenic substrates or internally quenched fluorescent peptides with specific inhibitors in the pH profile of proleolytic activity experiments in order to detect proteolytic activities in lysates of MDCK ...