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Effects of PSMA1 on the differentiation and lipid deposition of bovine preadipocytes
(Brazilian Journal of Animal Science, 2023)
Transfection of bovine fetal fibroblast with polyethylenimine (PEI) nanoparticles: effect of particle size and presence of fetal bovine serum on transgene delivery and cytotoxicity
(Springer, 2017-08)
The development of efficient transfection protocols for livestock cells is crucial for implementation of cell-based transgenic methods to produce genetically modified animals. We synthetized fully deacylated linear 22, 87 ...
Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIII
(London, 2011)
Abstract
Background
Hemophilia A is a bleeding disorder caused by deficiency in coagulation factor VIII. Recombinant factor ...
46 meganuclease i-SceI enhances stable transgene integration in cultured bovine fetal fibroblasts
(Csiro Publishing, 2012-12)
Production of genetically modified large animals through somatic cell nuclear transfer (SCNT) requires genetic manipulation of cultured cells, which are subsequently used as nuclear donors to generate a transgenic animal. ...
Optimization of polyethylenimine-mediated transient transfection using response surface methodology design
(Pontificia Universidad Católica de Valparaíso, 2010)
331 optimization of branched 25 kDa polyethylenimine for efficient gene delivery in bovine fetal fibroblasts
(Csiro Publishing, 2012-12)
Cost-effective, highly efficient, and noncytotoxic transfection of bovine fetal fibroblasts (BFF) has proven difficult to achieve by regular chemical and physical methods. The aims of this study were to evaluate transient ...
Phosphorylated tau potentiates Aβ-induced mitochondrial damage in mature neurons
(Academic Press Inc., 2014)
Geração e caracterização de linhagens de Trypanosoma cruzi expressando proteínas fluorescentes como ferramentas para pesquisa em Doença de Chagas
(Universidade Federal de Minas GeraisUFMG, 2007-10-18)
Stable transfection protocols have been described for a number of protozoan parasites such as Leishmania spp, Trypanosoma brucei and Trypanosoma cruzi. The transfection can be achieved by the integration of the foreign ...
Transfección de células por medio de ondas de choque
(Revista Mexicana de Física, 2009)